CMRL1066 w/L-Glutamine Culture Media
CMRL1066 w/L-Glutamine (Powder)
Purity
Cell Culture Grade
Synonyms
CMRL1066 w/L-Glutamine; N/A; CMRL1066 w/L-Glutamine (Powder); CMRL1066 w/L-Glutamine culture media
Purity/Purification
Cell Culture Grade
Application Notes
Hygroscopic. Keep tightly closed and dessicated.
Appearance
Light Orange, homogeneous, free flowing powder
Solubility
Yellow, clear, complete
Endotoxin Level
< 1EU/ml
pH
3.9
Directions per Liter
Dissolve 9.82g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components shown as g/liter
Inorganic Salts:
Calcium Chloride, 0.2
Magnesium Sulfate, 0.09769
Potassium Chloride 0.4
Sodium Acetate, 0.05
Sodium Chloride 6.8
Sodium Phosphate Monobasic, 0.122
Amino Acids
L-Alanine 0.025
L-Arginine 0.05787
L-Aspartic Acid 0.03
L-Cysteine•HCL•H2O 0.26
L-Cystine•2HCl 0.02
L-Glutamic Acid, Sodium 0.075
L-Glutamine 0.1
Glycine 0.05
L-Histidine•HCl•H2O 0.02
trans-4-Hydroxy-L-Proline 0.01
L-Isoleucine 0.02
L-Leucine 0.06
L-Lysine•HCl 0.07
L-Methionine 0.015
L-Phenylalanine 0.025
L-Proline 0.04
L-Serine 0.025
L-Threonine 0.03
L-Tryptophan 0.01
L-Tyrosine 0.04
L-Valine 0.025
Vitamins
PABA 0.00005
L-Ascorbic Acid•Na 0.05
D-Biotin 0.00001
Choline Chloride 0.0005
Coenzyme A•Na 0.0025
Cocarboxylase 0.001
2'-Deoxyadenosine 0.01
2'-Deoxycytidine•HCl 0.0116
2'-Deoxyguanosine 0.01
Folic Acid 0.00001
myo-Inositol 0.00005
5-Methyldeoxycytidine 0.0001
b-NAD 0.007
b-NADP•Na 0.001
Nicotinic Acid 0.000025
Niacinamide 0.000025
D-Pantothenic Acid•Ca 0.00001
Pyridoxal•HCl 0.000025
Pyridoxine•HCl 0.000025
Riboflavin 0.00001
Flavin Adenine Dinucleotide•2Na 0.000106
Thiamine•HCl 0.00001
Other
Cholesterol 0.0002
Thymidine 0.01
D-Glucose 1.00
D-GlucuronicAcid•Na 0.00388
Glutathione 0.01
Phenol Red,Sodium 0.02124
Tween 80 0.005
Uridine-5-Triphosphate•4Na 0.001
Total: 9.823g/liter
Calcium Chloride, 0.2
Magnesium Sulfate, 0.09769
Potassium Chloride 0.4
Sodium Acetate, 0.05
Sodium Chloride 6.8
Sodium Phosphate Monobasic, 0.122
Amino Acids
L-Alanine 0.025
L-Arginine 0.05787
L-Aspartic Acid 0.03
L-Cysteine•HCL•H2O 0.26
L-Cystine•2HCl 0.02
L-Glutamic Acid, Sodium 0.075
L-Glutamine 0.1
Glycine 0.05
L-Histidine•HCl•H2O 0.02
trans-4-Hydroxy-L-Proline 0.01
L-Isoleucine 0.02
L-Leucine 0.06
L-Lysine•HCl 0.07
L-Methionine 0.015
L-Phenylalanine 0.025
L-Proline 0.04
L-Serine 0.025
L-Threonine 0.03
L-Tryptophan 0.01
L-Tyrosine 0.04
L-Valine 0.025
Vitamins
PABA 0.00005
L-Ascorbic Acid•Na 0.05
D-Biotin 0.00001
Choline Chloride 0.0005
Coenzyme A•Na 0.0025
Cocarboxylase 0.001
2'-Deoxyadenosine 0.01
2'-Deoxycytidine•HCl 0.0116
2'-Deoxyguanosine 0.01
Folic Acid 0.00001
myo-Inositol 0.00005
5-Methyldeoxycytidine 0.0001
b-NAD 0.007
b-NADP•Na 0.001
Nicotinic Acid 0.000025
Niacinamide 0.000025
D-Pantothenic Acid•Ca 0.00001
Pyridoxal•HCl 0.000025
Pyridoxine•HCl 0.000025
Riboflavin 0.00001
Flavin Adenine Dinucleotide•2Na 0.000106
Thiamine•HCl 0.00001
Other
Cholesterol 0.0002
Thymidine 0.01
D-Glucose 1.00
D-GlucuronicAcid•Na 0.00388
Glutathione 0.01
Phenol Red,Sodium 0.02124
Tween 80 0.005
Uridine-5-Triphosphate•4Na 0.001
Total: 9.823g/liter
Preparation and Storage
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Related Product Information for CMRL1066 w/L-Glutamine culture media
CMRL-1066 is a chemically defined medium developed in the late 1960's at the Connaugh Medical Research Laboratories. CMRL-1066 was designed initially for use with mouse L-cells in non-supplemented culture. Although developed for use in serum-free culture, CMRL-1066 can be supplemented with serum and used to support the growth of many cell types. Suitable for growing a wide variety of cell types, especially fibroblast-like cells.
Product Categories/Family for CMRL1066 w/L-Glutamine culture media
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Product Notes
The CMRL1066 w/L-Glutamine (Catalog #AAA14830) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. Hygroscopic. Keep tightly closed and dessicated. Researchers should empirically determine the suitability of the CMRL1066 w/L-Glutamine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CMRL1066 w/L-Glutamine, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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