Human Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 ELISA Kit | CYP19A1 elisa kit

Human Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 ELISA Kit

Cat. Num. AAA96725

• Gene Names: CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• Reactivity: Human
• Synonyms: Cytochrome P450, Family 19, Subfamily A, Polypeptide 1; N/A; Human Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 ELISA Kit; CYP19A1 elisa kit

• Reactivity: Human
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CYP19A1 elisa kit

Principle of the assay: CYP19A1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CYP19A1. Standards or samples are then added to the microtiter plate wells and CYP19A1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CYP19A1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CYP19A1 are added to each well to “sandwich” the CYP19A1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CYP19A1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP19A1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13904860
• NCBI GeneID: 1588
• NCBI Accession #: NP_112503.1
• NCBI GenBank Nucleotide #: NM_031226.2
• Molecular Weight: 24,518 Da
• NCBI Official Full Name: aromatase
• NCBI Official Synonym Full Names: cytochrome P450, family 19, subfamily A, polypeptide 1
• NCBI Official Symbol: CYP19A1
• NCBI Official Synonym Symbols: ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• NCBI Protein Information: aromatase; estrogen synthase; estrogen synthetase; cytochrome P-450AROM; cytochrome P450 19A1; microsomal monooxygenase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily XIX (aromatization of androgens)
• UniProt Protein Name: Aromatase
• UniProt Gene Name: CYP19A1
• UniProt Synonym Gene Names: ARO1; CYAR; CYP19
• UniProt Entry Name: CP19A_HUMAN