Mouse cytochrome P450, family 19, subfamily A, polypeptide 1 ELISA Kit | CYP19A1 elisa kit

Mouse Aromatase, ARO ELISA Kit

Cat. Num. AAA79554

• Gene Names: Cyp19a1; Ar; ArKO; Int5; Cyp19; Int-5; p450arom
• Reactivity: Mouse
• Synonyms: cytochrome P450, family 19, subfamily A, polypeptide 1; N/A; Mouse Aromatase, ARO ELISA Kit; Mouse Aromatase (ARO) ELISA Kit; ARO; ARO1; CPV1; CYAR; CYP19; MGC104309; P-450AROM; OTTHUMP00000162543; aromatase; cytochrome P450; subfamily XIX (aromatization of androgens) ; estrogen synthetase; flavoprotein-linked monooxygenase; mic; family 19; subfamily A; polypeptide 1; CYP19A1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse ARO. No significant cross-reactivity or interference between mouse ARO and analogues was observed.
• Sequence Length: 503
• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 6.25 pg/ml-400 pg/ml.
• Sensitivity: The minimum detectable dose of mouse ARO is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for CYP19A1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ARO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ARO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ARO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ARO bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6681099
• NCBI GeneID: 13075
• NCBI Accession #: NP_031836.1
• NCBI GenBank Nucleotide #: NM_007810.3
• UniProt Accession #: P28649
• Molecular Weight: 58,015 Da
• NCBI Official Full Name: aromatase
• NCBI Official Synonym Full Names: cytochrome P450, family 19, subfamily a, polypeptide 1
• NCBI Official Symbol: Cyp19a1
• NCBI Official Synonym Symbols: Ar; ArKO; Int5; Cyp19; Int-5; p450arom
• NCBI Protein Information: aromatase; CYPXIX; estrogen synthase; cytochrome P-450AROM; cytochrome P450 19A1; cytochrome P450, 19, aromatase
• UniProt Protein Name: Aromatase
• UniProt Gene Name: Cyp19a1
• UniProt Synonym Gene Names: Arom; Cyp19
• UniProt Entry Name: CP19A_MOUSE