Standard Curve (Sample)
Human defensin, alpha 6, Paneth cell-specific ELISA Kit | DEFA6 elisa kit
Human Defensin-6, DEFA6 ELISA Kit
Gene Names
DEFA6; DEF6; HD-6
Reactivity
Human
Synonyms
defensin, alpha 6, Paneth cell-specific; N/A; Human Defensin-6, DEFA6 ELISA Kit; Human Defensin-6 (DEFA6) ELISA kit; DEF6; HD-6; defensin 6; defensin; alpha 6; Paneth cell-specific; DEFA6 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human DEFA6. No significant cross-reactivity or interference between human DEFA6 and analogues was observed.
Sequence Length
100
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.156 ng/ml -10 ng/ml.
Sensitivity
The minimum detectable dose of human DEFA6 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for DEFA6 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFA6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFA6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFA6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFA6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,975 Da
NCBI Official Full Name
defensin-6 preproprotein
NCBI Official Synonym Full Names
defensin, alpha 6, Paneth cell-specific
NCBI Official Symbol
DEFA6
NCBI Official Synonym Symbols
DEF6; HD-6
NCBI Protein Information
defensin-6; defensin 6
UniProt Protein Name
Defensin-6
UniProt Gene Name
DEFA6
UniProt Synonym Gene Names
DEF6
UniProt Entry Name
DEF6_HUMAN
Similar Products
Product Notes
The Human DEFA6 defa6 (Catalog #AAA113033) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA113033 ELISA Kit recognizes Human DEFA6. It is sometimes possible for the material contained within the vial of "defensin, alpha 6, Paneth cell-specific, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.