Annexin V Detection Kit

Annexin V-APC/PI Apoptosis Detection Kit

Cat. Num. AAA174685

• Synonyms: Annexin V; N/A; Annexin V-APC/PI Apoptosis Detection Kit; Annexin V detection kit

• Related Products: Annexin V-FITC Reagent ; Annexin V-AF647 Reagent ; Annexin V-PE Reagent ; Annexin V-APC Reagent ; Annexin V-EGFP Reagent ; Annexin V Binding Buffer(10 ×) ; Propidium Iodide (PI) Staining Solution ; 7-AAD Viability Staining Solution ; Annexin V-FITC / PI Apoptosis Detection Kit ; Annexin V-FITC / 7-AAD Apoptosis Detection Kit ; Annexin V-AF647 / PI Apoptosis Detection Kit ; Annexin V-AF647 / 7-AAD Apoptosis Detection Kit ; Annexin V-PE / 7-AAD Apoptosis Detection Kit ; Annexin V-APC / 7-AAD Apoptosis Detection Kit ; Annexin V-EGFP / PI Apoptosis Detection Kit ; Annexin V-EGFP / 7-AAD Apoptosis Detection Kit

• Instructions: The Annexin V Binding Buffer (10 ×)[Cat: MBS2557011] is a 10 × concentrated solution. Dilute with DI water to 1 × working solution before use. ; For example: Take 1 mL Annexin V Binding Buffer (10 ×), dilute with DI water to 10 mL.
• Staining Procedure: 1. Induce apoptosis of suspension cells with reagents of interest. Collect cell cultures and centrifuge at 300 g for 5 min, discard the supernatant. Add appropriate PBS to wash the cells, resuspend gently and count the cells. ; Note: This product is only validated in suspension cells. Good cell viability is the key to the experiment. When the adherent cells are used for apoptotic detection, treatments like digestion may increase the ratio of necrotic or apoptotic cells and cause uncontrollable effects on the experimental results. Please be aware!; 2. Split the cell suspension into tubes, 1-5 × 105 cells for each. Centrifuge at 300 g for 5 min, discard the supernatant. Add appropriate PBS to wash the cells and discard the supernatant. Add 500 uL of 1 × Annexin V Binding Buffer to resuspend the cells.; 3. Add 5 ul of Annexin V-APC and 5 ul of Propidium Iodide (PI) Staining Solution to each tube.; 4. Gently vortex the cells and incubate at room temperature for 15-20 min in the dark.; 5. Analyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.
• Preparation and Storage: Store at 4°C for up to one year. Annexin V-APC and PI Staining Solution should be stored in dark.; Cautions:; 1. For maximal assay performance, this kit should be used within 3-6 months. Avoid freeze / thaw cycles.; 2. Detect apoptosis as soon as possible after staining to avoid the increase number of apoptosis or necrosis.; 3. Avoid extended exposure of the samples to direct light to protect the fluorophores from quenching.; 4. For your safety and health, please wear the lab coat and disposable gloves before the experiments.

Application Data

(Jurkat cells were treated with 1uM Camptothecin and detected by this kit)

Related Product Information for Annexin V detection kit

Annexin V-APC/PI Cell Apoptosis Detection kit is developed to identify apoptotic and necrotic cells.
Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The fluorescent format of this intracellular protein, Annexin V-APC, binds specifically to the PS on outer leaflet of cell membrane by flow cytometry or fluorescence microscopy.
Propidium Iodide (PI) is a common DNA dye that is not permeable to cell membrane. Once binding to DNA, the flurescence of PI increases by nearly 20 fold. Due to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA. Cells at different apoptotic stages can be distinguished by using Annexin V and PI.

Product Categories/Family for Annexin V detection kit

Apoptosis Detection Kit