Fluorescent Phosphate Detection Kit
Fluorescent Phosphate Detection Kit
Synonyms
Fluorescent Phosphate; N/A; Fluorescent Phosphate Detection Kit; Fluoro Phosphate Detection Kit; Fluorescent Phosphate detection kit
Excitation/Emission
535/585nm or Absorbance readout at 570nm
Method of Analysis
Fluorescence plate reader or spectrophotometer
Preparation and Storage
Store contents as labeled
Shipping: Blue Ice/Gel Packs
Shipping: Blue Ice/Gel Packs
Related Product Information for Fluorescent Phosphate detection kit
This kit provides a simple, one-step fluorometric or colorimetric method for determination of phosphate in serum and plasma samples. The assay is based on an enzyme-coupled reaction that detects inorganic phosphate.
Phosphorus is essential for multiple and diverse biological functions, including cellular signal transduction, mineral metabolism, and energy exchange. Although >80% of total body phosphorus is stored in bone and teeth, intracellular phosphorus exists in the form of organic compounds such as ATP and as free anions like H3PO43-, which are commonly referred to as phosphate. Serum phosphorus primarily occurs in the form of inorganic phosphate, which is maintained within the physiological range by regulation of dietary absorption, bone formation, and renal excretion, as well as equilibrium with intracellular stores. Elevated levels of serum phosphate may be associated with a higher risk of death and adverse cardiovascular outcomes in people with prior myocardial infarction, and is the subject of large amount of research. Rapid and easy quantification of serum phosphate in experimental samples may prove clinically useful.
The Fluorescent Phosphate Detection Kit provides a simple, one-step fluorometric or colorimetric method for determination of phosphate in serum and plasma samples. The assay is based on an enzyme-coupled reaction that detects inorganic phosphate. The substrate is converted to Hydrogen peroxide in presence of inorganic phosphate in a coupled enzymatic reaction. The Hydrogen Peroxide then reacts with the detection reagent in a 1:1 stoichiometry in presence of Horse Radish Peroxidase to produce the stable fluorescent product.
Phosphorus is essential for multiple and diverse biological functions, including cellular signal transduction, mineral metabolism, and energy exchange. Although >80% of total body phosphorus is stored in bone and teeth, intracellular phosphorus exists in the form of organic compounds such as ATP and as free anions like H3PO43-, which are commonly referred to as phosphate. Serum phosphorus primarily occurs in the form of inorganic phosphate, which is maintained within the physiological range by regulation of dietary absorption, bone formation, and renal excretion, as well as equilibrium with intracellular stores. Elevated levels of serum phosphate may be associated with a higher risk of death and adverse cardiovascular outcomes in people with prior myocardial infarction, and is the subject of large amount of research. Rapid and easy quantification of serum phosphate in experimental samples may prove clinically useful.
The Fluorescent Phosphate Detection Kit provides a simple, one-step fluorometric or colorimetric method for determination of phosphate in serum and plasma samples. The assay is based on an enzyme-coupled reaction that detects inorganic phosphate. The substrate is converted to Hydrogen peroxide in presence of inorganic phosphate in a coupled enzymatic reaction. The Hydrogen Peroxide then reacts with the detection reagent in a 1:1 stoichiometry in presence of Horse Radish Peroxidase to produce the stable fluorescent product.
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Product Notes
The Fluorescent Phosphate (Catalog #AAA52789) is a Detection Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Fluorescent Phosphate, Detection Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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