WB (Western Blot)
(Western blot analysis of lysates of transfected HEK293 using rabbit pAb to GFP (in green), dilution 1:2,000. [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct.)
Rabbit Green Fluorescent Protein (GFP) Polyclonal Antibody | anti-GFP antibody
Green Fluorescent Protein (GFP)
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
Green Fluorescent Protein (GFP), Antibody; Green Fluorescent Protein (GFP); anti-GFP antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Form/Format
Purified liquid antibody in 50% PBS, 50% glycerol plus 5mM of Sodium Azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GFP antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant Green Fluorescent Protein purified from E Coli
Preparation and Storage
Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2-8 degree C for 1 month without detectable loss ofactivity. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates of transfected HEK293 using rabbit pAb to GFP (in green), dilution 1:2,000. [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct.)
WB (Western Blot)
(Western blot analysis of lysates of transfected HEK293 using rabbit pAb to GFP (in green), dilution 1:2,000. [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct.)
IF (Immunofluorescence)
(Immunofluorescent analysis of transfected HEK293 cells with a GFP construct, in green, and stained with rabbit pAb to GFP, RA22134, dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The GFP antibody reveals GFP protein expressed only in transfected cells, and as a result these cells appear orange-yellow in color.)
IF (Immunofluorescence)
(Immunofluorescent analysis of transfected HEK293 cells with a GFP construct, in green, and stained with rabbit pAb to GFP, RA22134, dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The GFP antibody reveals GFP protein expressed only in transfected cells, and as a result these cells appear orange-yellow in color.)
Related Product Information for anti-GFP antibody
GFP (green fluorescent protein) is 27kDa protein; which was original isolated from the jellyfish (Aequoria victoria). This antibody was made against a recombinant GFP, from the Aequoria species, referred to as AcGFP. This antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.
NCBI and Uniprot Product Information
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Product Notes
The GFP gfp (Catalog #AAA76689) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Green Fluorescent Protein (GFP) can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GFP gfp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Green Fluorescent Protein (GFP), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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