ELISA
Protein-Free Sample Diluent
Protein-Free Sample Diluent
Synonyms
Protein-Free Sample Diluent; N/A; Protein-Free Sample Diluent diluent
Specifications
• Clear liquid
• 1X ready to use
• 1X ready to use
pH
7.1-7.6
Instructions
1. Prepare standards in Protein-Free Sample Diluent.
2. Dilute samples in Protein-Free Sample Diluent.
• Serum samples should generally be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding.
• To dilute the sample 1:100, add 1 part sample to 99 parts Protein-Free Sample Diluent. For example, add 10 uL sample to 990 uL Protein-Free Sample Diluent for a total of 1,000 uL.
• Highly concentrated samples may need to be diluted 1:1,000 or more.
3. Once diluted, run the assay according to the spe-cific ELISA protocol.
4. Analyze the data. If the samples were diluted 1:100, the dilution factor must be considered when calculating the value. For example, if the sample generated an OD value that correlates to 500 pg/mL based on the standard curve, multiply by the dilution factor of 100 to yield a true concentration of 50,000 pg/mL = 50 ng/mL in the sample.
2. Dilute samples in Protein-Free Sample Diluent.
• Serum samples should generally be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding.
• To dilute the sample 1:100, add 1 part sample to 99 parts Protein-Free Sample Diluent. For example, add 10 uL sample to 990 uL Protein-Free Sample Diluent for a total of 1,000 uL.
• Highly concentrated samples may need to be diluted 1:1,000 or more.
3. Once diluted, run the assay according to the spe-cific ELISA protocol.
4. Analyze the data. If the samples were diluted 1:100, the dilution factor must be considered when calculating the value. For example, if the sample generated an OD value that correlates to 500 pg/mL based on the standard curve, multiply by the dilution factor of 100 to yield a true concentration of 50,000 pg/mL = 50 ng/mL in the sample.
Preparation and Storage
Store at 2-8°C.
ELISA
ELISA
Related Product Information for Protein-Free Sample Diluent diluent
Protein-Free Sample Diluent is a protein-free matrix used for the dilution of biological samples (e.g. serum, cell culture media) into the useful range of antibody-sandwich or antigen-down ELISA format assays. This unique buffer contains a heterogeneous mixture of proprietary molecules that help reduce background noise associated with non-specific bridging of signal-generating conjugates to the plate well surface.
Sample diluents are used to dilute samples into the functional range of the assay and to create the standard curve. Due to the finite binding capacity of the plate well-coated proteins (e.g., antibodies, antigens), highly concentrated samples must be diluted in order to obtain absorbance readings within the sensitivity detection limits of the instrument. Properly formulated sample diluents will also reduce background noise associated with non-specific bridging of signal-generating conjugates to the plate well surface.
Protein-Free Sample Diluent provides a non-protein-based buffered, neutral pH environment that is highly compatible with antibody-antigen interactions. Antimicrobial agents allow for room temperature bench-top use and extensive storage stability at 2-8°C.
Sample diluents are used to dilute samples into the functional range of the assay and to create the standard curve. Due to the finite binding capacity of the plate well-coated proteins (e.g., antibodies, antigens), highly concentrated samples must be diluted in order to obtain absorbance readings within the sensitivity detection limits of the instrument. Properly formulated sample diluents will also reduce background noise associated with non-specific bridging of signal-generating conjugates to the plate well surface.
Protein-Free Sample Diluent provides a non-protein-based buffered, neutral pH environment that is highly compatible with antibody-antigen interactions. Antimicrobial agents allow for room temperature bench-top use and extensive storage stability at 2-8°C.
Product Categories/Family for Protein-Free Sample Diluent diluent
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Product Notes
The Protein-Free Sample Diluent (Catalog #AAA52767) is a Diluent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Protein-Free Sample Diluent, Diluent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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