Application Data
Plasma Sample Diluent
Plasma Sample Diluent (Antigen-Down SD)
Synonyms
Plasma Sample Diluent; N/A; Plasma Sample Diluent (Antigen-Down SD); Plasma Sample Diluent diluent
Form/Format
Clear to light yellow liquid
pH
7.2-7.6
Instructions
1. Generally, serum samples should be diluted at least 1:50 to minimize background noise caused by non-specific antibody binding.
2. To dilute the sample 1:100, add 1 part sample to 99 parts Plasma Sample Diluent. For example, add 10 uL sample to 990 uL Plasma Sample Diluent for a total of 1,000 uL.
3. Highly concentrated samples may need to be diluted 1:1,000 or more.
4. Once diluted, run the assay according to the specific ELISA protocol.
5. Analyze the data. If the sample was diluted, the dilution factor must be considered when calculating the value.
2. To dilute the sample 1:100, add 1 part sample to 99 parts Plasma Sample Diluent. For example, add 10 uL sample to 990 uL Plasma Sample Diluent for a total of 1,000 uL.
3. Highly concentrated samples may need to be diluted 1:1,000 or more.
4. Once diluted, run the assay according to the specific ELISA protocol.
5. Analyze the data. If the sample was diluted, the dilution factor must be considered when calculating the value.
Preparation and Storage
24 months at 2-8°C
1 week at room temperature
1 week at room temperature
Application Data
Application Data
Related Product Information for Plasma Sample Diluent diluent
Plasma Sample Diluent is formulated to provide a stable protein-friendly environment for dilution of neat plasma or serum samples for analysis in antibodysandwich or antigen-down format ELISAs. It was specifically developed to provide a reliable solution to the occurrence of unwanted clotting events in plasma and serum samples within the microtiter plate wells. When assay sensitivity is a large concern, serum or plasma samples must be added to the ELISA plate neat or diluted only slightly, making clotting more likely. This issue can be essentially eliminated by diluting the potentially problematic samples 1:2 in Plasma Sample Diluent.
Sample diluents are used to dilute samples into the functional range of the assay and to create the standard curve. Due to the finite binding capacity of the plate-coated proteins, highly concentrated samples must be diluted in order to obtain absorbance readings within the sensitivity detection limits of the instrument. Properly formulated sample diluents will also reduce background noise associated with non-specific bridging of signal-generating conjugates to the microtiter plate surface.
Plasma Sample Diluent provides a mammalian protein-buff ered, neutral pH environment that is highly compatible with antibody-antigen interactions. Inclusion of a protein component helps minimize the degree of non-specific IgG adsorption onto coated and blocked ELISA plate wells during antigen-down screening of serum or plasma samples. Antimicrobial agents allow for room temperature bench-top use and extensive storage stability at 2-8°C.
Sample diluents are used to dilute samples into the functional range of the assay and to create the standard curve. Due to the finite binding capacity of the plate-coated proteins, highly concentrated samples must be diluted in order to obtain absorbance readings within the sensitivity detection limits of the instrument. Properly formulated sample diluents will also reduce background noise associated with non-specific bridging of signal-generating conjugates to the microtiter plate surface.
Plasma Sample Diluent provides a mammalian protein-buff ered, neutral pH environment that is highly compatible with antibody-antigen interactions. Inclusion of a protein component helps minimize the degree of non-specific IgG adsorption onto coated and blocked ELISA plate wells during antigen-down screening of serum or plasma samples. Antimicrobial agents allow for room temperature bench-top use and extensive storage stability at 2-8°C.
Product Categories/Family for Plasma Sample Diluent diluent
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Product Notes
The Plasma Sample Diluent (Catalog #AAA52765) is a Diluent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Plasma Sample Diluent, Diluent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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