Application Data
(Recombinant DNMT3B protein activity assay Recombinant DNMT3B protein activity was measured using DNMT Activity / Inhibition Assay, for 1 hour at 37 degree C)
DNMT3B recombinant protein
Recombinant DNMT3B protein
Gene Names
DNMT3B; ICF; ICF1; M.HsaIIIB
Purity
The recombinant protein is >60% pure by SDS-PAGE.
Synonyms
DNMT3B; N/A; Recombinant DNMT3B protein; ICF; ICF1; M.HsaIIIB; DNMT3B recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >60% pure by SDS-PAGE.
Form/Format
Full length recombinant DNMT3B protein is supplied in 25mM Tris pH8.0, 300mM NaCl, 5% Glycerol and 0.04% Triton X-100, 1mM DTT. Please refer to product insert upon arrival for lot-specific concentration.
Protein Species
Human
Tag
His-Tag
Notes
Recombinant DNMT3B protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. DNMT Activity Assay Conditions: Assay was performed using DNMT Activity/Inhibition Assay, for 1 hour at 37 degree C.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Recombinant DNMT3B protein activity assay Recombinant DNMT3B protein activity was measured using DNMT Activity / Inhibition Assay, for 1 hour at 37 degree C)
Application Data
(Recombinant DNMT3B protein activity assay Recombinant DNMT3B protein activity was measured using DNMT Activity / Inhibition Assay, for 1 hour at 37 degree C)
SDS-PAGE
(Recombinant DNMT3B protein gel. Recombinant DNMT3B protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)
SDS-PAGE
(Recombinant DNMT3B protein gel. Recombinant DNMT3B protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)
Related Product Information for DNMT3B recombinant protein
Short Description: Full-length recombinant DNMT3B protein (accession number NP_787045.1) was expressed in Sf9 cells and contains an N-terminal 6xHis-tag with a molecular weight of 89.7 kDa. The recombinant protein is >60% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 Beta) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3. The DNMT3 family contains two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L). DNMT3B has been shown to be important in the regulation of specific patterns of DNA methylation, specifically de novo methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression. Mutations in the DNMT3B gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome.
Background: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 Beta) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3. The DNMT3 family contains two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L). DNMT3B has been shown to be important in the regulation of specific patterns of DNA methylation, specifically de novo methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression. Mutations in the DNMT3B gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome.
Product Categories/Family for DNMT3B recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
81,311 Da
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 3B isoform 3
NCBI Official Synonym Full Names
DNA methyltransferase 3 beta
NCBI Official Symbol
DNMT3B
NCBI Official Synonym Symbols
ICF; ICF1; M.HsaIIIB
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 3B
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 3B
UniProt Gene Name
DNMT3B
UniProt Synonym Gene Names
Dnmt3b; DNA MTase HsaIIIB; M.HsaIIIB
UniProt Entry Name
DNM3B_HUMAN
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Product Notes
The DNMT3B dnmt3b (Catalog #AAA59665) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "DNMT3B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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