Human Anti double stranded DNA ELISA Kit | dsDNA elisa kit
Human Anti double stranded DNA ELISA Kit
Reactivity
Human
Synonyms
Anti double stranded DNA; N/A; Human Anti double stranded DNA ELISA Kit; dsDNA elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Anti-dsDNA. No significant cross-reactivity or interference between Anti-dsDNA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Anti-dsDNA and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for dsDNA elisa kit
Principle of the Assay: Anti-dsDNA ELISA kit applies the competitive enzyme immunoassay technique utilizing double stranded DNA and an Anti-dsDNA-HRP conjugate. The assay sample and buffer are incubated together with Anti-dsDNA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-dsDNA concentration since Anti-dsDNA from samples and Anti-dsDNA-HRP conjugate compete for the double stranded DNA binding site. Since the number of sites is limited, as more sites are occupied by Anti-dsDNA from the sample, fewer sites are left to bind Anti-dsDNA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-dsDNA concentration in each sample is interpolated from this standard curve.
Intended Uses: This Anti-dsDNA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Anti-dsDNA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This Anti-dsDNA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Anti-dsDNA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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