Human Anti double stranded DNA ELISA Kit | dsDNA elisa kit

Human Anti double stranded DNA ELISA Kit

Cat. Num. AAA16285

• Reactivity: Human
• Synonyms: Anti double stranded DNA; N/A; Human Anti double stranded DNA ELISA Kit; dsDNA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Anti-dsDNA. No significant cross-reactivity or interference between Anti-dsDNA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Anti-dsDNA and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for dsDNA elisa kit

Principle of the Assay: Anti-dsDNA ELISA kit applies the competitive enzyme immunoassay technique utilizing double stranded DNA and an Anti-dsDNA-HRP conjugate. The assay sample and buffer are incubated together with Anti-dsDNA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-dsDNA concentration since Anti-dsDNA from samples and Anti-dsDNA-HRP conjugate compete for the double stranded DNA binding site. Since the number of sites is limited, as more sites are occupied by Anti-dsDNA from the sample, fewer sites are left to bind Anti-dsDNA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-dsDNA concentration in each sample is interpolated from this standard curve.

Intended Uses: This Anti-dsDNA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Anti-dsDNA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for dsDNA elisa kit

Epigenetics and Nuclear Signaling