Bioactivity
(The cluster of differentiation (CD) antigen CD28 is a member of the immunoglobulin subfamily. CD28 is a central co-stimulatory molecule for TCR-mediated activation such as cytokine production and T-cell proliferation upon ligand binding and TCR stimulation. CD80 and CD86 are expressed on antigen presenting cells and CD86 is primary ligand for CD28. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat CD28 and recombinant human CD86. Briefly, CD28 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CD86-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CD28 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat CD28 and recombinant human CD86 was shown in Figure 1, the EC50 for this effect is 0.07 ug/mL.)
Cluster Of Differentiation 28 (CD28) Active Protein | CD28 active protein
Active Cluster Of Differentiation 28 (CD28)
Gene Names
Cd28; CD28RNA
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
Cluster Of Differentiation 28 (CD28); N/A; Active Cluster Of Differentiation 28 (CD28); Cluster Of Differentiation 28; Tp44; T-Cell-Specific Surface Glycoprotein4; CD28 active protein
Host
E Coli
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Asn20~Leu150
Applicable Applications for CD28 active protein
Activity Assay, Cell Culture
Species
Rat
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
6.5
Research Area
CD & Adhesion molecule
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(The cluster of differentiation (CD) antigen CD28 is a member of the immunoglobulin subfamily. CD28 is a central co-stimulatory molecule for TCR-mediated activation such as cytokine production and T-cell proliferation upon ligand binding and TCR stimulation. CD80 and CD86 are expressed on antigen presenting cells and CD86 is primary ligand for CD28. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat CD28 and recombinant human CD86. Briefly, CD28 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CD86-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CD28 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat CD28 and recombinant human CD86 was shown in Figure 1, the EC50 for this effect is 0.07 ug/mL.)
Bioactivity
(The cluster of differentiation (CD) antigen CD28 is a member of the immunoglobulin subfamily. CD28 is a central co-stimulatory molecule for TCR-mediated activation such as cytokine production and T-cell proliferation upon ligand binding and TCR stimulation. CD80 and CD86 are expressed on antigen presenting cells and CD86 is primary ligand for CD28. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat CD28 and recombinant human CD86. Briefly, CD28 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CD86-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CD28 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat CD28 and recombinant human CD86 was shown in Figure 1, the EC50 for this effect is 0.07 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for CD28 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,170 Da
NCBI Official Full Name
T-cell-specific surface glycoprotein CD28
NCBI Official Synonym Full Names
Cd28 molecule
NCBI Official Symbol
Cd28
NCBI Official Synonym Symbols
CD28RNA
NCBI Protein Information
T-cell-specific surface glycoprotein CD28; CD28 antigen
UniProt Protein Name
T-cell-specific surface glycoprotein CD28
UniProt Gene Name
Cd28
UniProt Entry Name
CD28_RAT
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Product Notes
The CD28 cd28 (Catalog #AAA161758) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cluster Of Differentiation 28 (CD28) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the CD28 cd28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Asn20~Leu1 50. It is sometimes possible for the material contained within the vial of "Cluster Of Differentiation 28 (CD28), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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