Bioactivity
(Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human M-CSF and recombinant human Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra). Briefly, biotin-linked M-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CSF2Ra-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human M-CSF and recombinant human CSF2Ra was shown in Figure 1, the EC50 for this effect is 0.02 ug/mL.)
Colony Stimulating Factor 1, Macrophage (MCSF) Active Protein | MCSF active protein
Active Colony Stimulating Factor 1, Macrophage (MCSF)
Gene Names
CSF1; MCSF; CSF-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Synonyms
Colony Stimulating Factor 1, Macrophage (MCSF); N/A; Active Colony Stimulating Factor 1, Macrophage (MCSF); Colony Stimulating Factor 1, Macrophage; CSF1; M-CSF; Lanimostim; Processed macrophage colony-stimulating factor 14; MCSF active protein
Host
E Coli
Purity/Purification
Greater than 95% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Glu33~Asn190
Applicable Applications for MCSF active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
4.6
Research Area
Cytokine, Infection immunity
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human M-CSF and recombinant human Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra). Briefly, biotin-linked M-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CSF2Ra-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human M-CSF and recombinant human CSF2Ra was shown in Figure 1, the EC50 for this effect is 0.02 ug/mL.)
Bioactivity
(Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human M-CSF and recombinant human Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra). Briefly, biotin-linked M-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CSF2Ra-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human M-CSF and recombinant human CSF2Ra was shown in Figure 1, the EC50 for this effect is 0.02 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for MCSF active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
60,179 Da
NCBI Official Full Name
colony-stimulating factor (CSF-1)
NCBI Official Synonym Full Names
colony stimulating factor 1 (macrophage)
NCBI Official Symbol
CSF1
NCBI Official Synonym Symbols
MCSF; CSF-1
NCBI Protein Information
macrophage colony-stimulating factor 1; lanimostim
UniProt Protein Name
Macrophage colony-stimulating factor 1
UniProt Gene Name
CSF1
UniProt Synonym Gene Names
CSF-1; M-CSF; MCSF
UniProt Entry Name
CSF1_HUMAN
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Product Notes
The MCSF csf1 (Catalog #AAA161683) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Colony Stimulating Factor 1, Macrophage (MCSF) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the MCSF csf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Glu33~Asn1 90. It is sometimes possible for the material contained within the vial of "Colony Stimulating Factor 1, Macrophage (MCSF), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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