SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Cyclophilin A Active Protein | PPIA active protein
Cyclophilin A, 1-165aa, Human, E Coli (Bioactivity Validated)
Gene Names
PPIA; CYPA; CYPH; HEL-S-69p
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
Cyclophilin A; N/A; Cyclophilin A, 1-165aa, Human, E Coli (Bioactivity Validated); Peptidylprolyl isomerase A; CYPA; CYPH; MGC12404; MGC23397; MGC117158; Cyclosporin A binding protein; Peptidyl prolyl cis trans isomerase A; PPIA; PPIA protein; PPIase A; Rotamase A; RotamaseA; T cell cyclophilin.; PPIA active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
In 20mM Tris pH 8.0, .20mM NaCl, 0.5mM DTT, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence Positions
1-165aa
Sequence
MGSSHHHHHH SSGLVPRGSH MVNPTVFFDI AVDGEPLGRV SFELFADKVP KTAENFRALS TGEKGFGYKG SCFHRIIPGF MCQGGDFTRH NGTGGKSIYG EKFEDENFIL KHTGPGILSM ANAGPNTNGS QFFICTAKTE WLDGKHVVFG KVKEGMNIVE AMERFGSRNG KTSKKITIAD CGQLE
Applicable Applications for PPIA active protein
Enzyme Activity, SDS-PAGE
Antigen Species
Human
Tag
His-Tag
Endotoxin Level
< 1.0 EU per 1ug of protein (determined by LAL method)
Biological Activity
Specific activity is > 650 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Preparation and Storage
Can be stored at 2-8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for PPIA active protein
Cyclophilin A (also known as Peptidylprolyl isomerase A, PPIA) encodes a member of the peptidyl-prolyl cis-trans isomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. Cyclophilin A is also incorporated into many viruses, including HIV-1, where it has been speculated to be involved in functions such as viral assembly and infectivity. Recombinant human PPIA, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for PPIA active protein
References
Holzman, T.F., et al, (1991). J. Biol. Chem. 266 (4): 2474-2479; Berthoux L, et al, (2005). Proc Natl Acad Sci u S A. 102 (41):14849-53;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20kDa (185aa), confirmed by MALDI-TOF.
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase A isoform 1
NCBI Official Synonym Full Names
peptidylprolyl isomerase A
NCBI Official Symbol
PPIA
NCBI Official Synonym Symbols
CYPA; CYPH; HEL-S-69p
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase A
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase A
UniProt Gene Name
PPIA
UniProt Synonym Gene Names
CYPA; PPIase A
Similar Products
Product Notes
The PPIA ppia (Catalog #AAA48477) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-165aa. AAA Biotech's Cyclophilin A can be used in a range of immunoassay formats including, but not limited to, Enzyme Activity, SDS-PAGE. Researchers should empirically determine the suitability of the PPIA ppia for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MVNPTVFFDI AVDGEPLGRV SFELFADKVP KTAENFRALS TGEKGFGYKG SCFHRIIPGF MCQGGDFTRH NGTGGKSIYG EKFEDENFIL KHTGPGILSM ANAGPNTNGS QFFICTAKTE WLDGKHVVFG KVKEGMNIVE AMERFGSRNG KTSKKITIAD CGQLE. It is sometimes possible for the material contained within the vial of "Cyclophilin A, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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