WB (Western Blot)
(Anti-PPIA rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: NIH-3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:15 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human Cyclophilin A Polyclonal Antibody | anti-PPIA antibody
Anti-Cyclophilin A Antibody, Rabbit Polyclonal
Gene Names
PPIA; CYPA; CYPH
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Cyclophilin A, Antibody; Anti-Cyclophilin A Antibody, Rabbit Polyclonal; Cyclophilin A Antibody, Rabbit PAb, Antigen Affinity Purified; peptidylprolyl isomerase A (cyclophilin A); Anti-CYPA Antibody; Anti-CYPH Antibody; Anti-HEL-S-69p Antibody; anti-PPIA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Cyclophilin A
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PPIA antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Human Cyclophilin A protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human Cyclophilin A (rh Cyclophilin A). Cyclophilin A specific IgG was purified by Human Cyclophilin A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-PPIA rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: NIH-3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:15 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-PPIA rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: NIH-3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:15 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(PPIA was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate2 uL anti-PPIA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PPIA rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 16 kDa)
IP (Immunoprecipitation)
(PPIA was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate2 uL anti-PPIA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PPIA rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 16 kDa)
Related Product Information for anti-PPIA antibody
Peptidyl-prolyl cis-trans isomerase A, also known as PPIase A, Rotamase A, Cyclophilin A, Cyclosporin A-binding protein, PPIA and CYPA, is a cytoplasm protein that belongs to the cyclophilin-type PPIase family and PPIase A subfamily. Cyclophilins (CyPs) are a family of proteins found in organisms ranging from prokaryotes to humans. These molecules exhibit peptidyl-prolyl isomerase activity, suggesting that they influence the conformation of proteins in cells. PPIA/Cyclophilin A accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA/Cyclophilin A is secreted by vascular smooth muscle cells in response to inflammatory stimuli, and could thus contribute to atherosclerosis. It is not essential for mammalian cell viability. PPIA/Cyclophilin A can interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions.
Product Categories/Family for anti-PPIA antibody
References
Haendler B., et al., (1987), Complementary DNA for human T-cell cyclophilin. EMBO J. 6:947-950.Haendler B., et al., (1990), Characterization of the human cyclophilin gene and of related processed pseudogenes.Eur. J. Biochem. 190:477-482.Ota T., et al., (2004), Complete sequencing and characterization of 21, 243 full-length human cDNAs.Nat. Genet. 36:40-45.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
165
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase A
NCBI Official Synonym Full Names
peptidylprolyl isomerase A (cyclophilin A)
NCBI Official Symbol
PPIA
NCBI Official Synonym Symbols
CYPA; CYPH
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase A; PPIase A; rotamase A; cyclophilin; T cell cyclophilin; cyclosporin A-binding protein
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase A
UniProt Gene Name
PPIA
UniProt Synonym Gene Names
CYPA; PPIase A
UniProt Entry Name
PPIA_HUMAN
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Product Notes
The PPIA ppia (Catalog #AAA254928) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cyclophilin An Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclophilin A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PPIA ppia for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclophilin A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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