Bioactivity
(Ephrin A3 (EFNA3), also known as EFL2, EPLG3, LERK3, Ehk1-L, is a member of the Eph/ephrin tyrosine kinase family. EFNA3 is localized in cell membranes and was highly expressed in brain, skin, esophagus, skeletal muscle, spleen, thymus, prostate, testis, ovary, small intestine, peripheral blood leukocytes. EFNA3, like most genes in the ephrin family, plays a central role in embryonic development can associate with multiple signaling pathways involved in cell growth and tumor cell metastasis. Aberrant regulation of EFNA3 is associated with the occurrence and development of various types of cancer. Ephrin Type A Receptor 4 (EPHA4) is a receptor for members of the ephrin-A family. It can bind to EFNA3. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EFNA3 and recombinant human EPHA4. Briefly, EFNA3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to EPHA4-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-EFNA3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human EFNA3 and recombinant human EPHA4 was shown in Figure 1, the EC50 for this effect is 0.4 ug/mL.)
Ephrin A3 (EFNA3) Active Protein | EFNA3 active protein
Active Ephrin A3 (EFNA3)
Gene Names
EFNA3; EFL2; EPLG3; LERK3; Ehk1-L
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
Ephrin A3 (EFNA3); N/A; Active Ephrin A3 (EFNA3); Ephrin A3; EFL2; EPLG3; Ehk1-L; LERK3; EHK1 ligand; EPH-related receptor tyrosine kinase ligand 34; EFNA3 active protein
Host
E Coli
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Gly30~Ser238
Applicable Applications for EFNA3 active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
8.9
Research Area
Neuro science
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Ephrin A3 (EFNA3), also known as EFL2, EPLG3, LERK3, Ehk1-L, is a member of the Eph/ephrin tyrosine kinase family. EFNA3 is localized in cell membranes and was highly expressed in brain, skin, esophagus, skeletal muscle, spleen, thymus, prostate, testis, ovary, small intestine, peripheral blood leukocytes. EFNA3, like most genes in the ephrin family, plays a central role in embryonic development can associate with multiple signaling pathways involved in cell growth and tumor cell metastasis. Aberrant regulation of EFNA3 is associated with the occurrence and development of various types of cancer. Ephrin Type A Receptor 4 (EPHA4) is a receptor for members of the ephrin-A family. It can bind to EFNA3. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EFNA3 and recombinant human EPHA4. Briefly, EFNA3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to EPHA4-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-EFNA3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human EFNA3 and recombinant human EPHA4 was shown in Figure 1, the EC50 for this effect is 0.4 ug/mL.)
Bioactivity
(Ephrin A3 (EFNA3), also known as EFL2, EPLG3, LERK3, Ehk1-L, is a member of the Eph/ephrin tyrosine kinase family. EFNA3 is localized in cell membranes and was highly expressed in brain, skin, esophagus, skeletal muscle, spleen, thymus, prostate, testis, ovary, small intestine, peripheral blood leukocytes. EFNA3, like most genes in the ephrin family, plays a central role in embryonic development can associate with multiple signaling pathways involved in cell growth and tumor cell metastasis. Aberrant regulation of EFNA3 is associated with the occurrence and development of various types of cancer. Ephrin Type A Receptor 4 (EPHA4) is a receptor for members of the ephrin-A family. It can bind to EFNA3. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EFNA3 and recombinant human EPHA4. Briefly, EFNA3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to EPHA4-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-EFNA3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human EFNA3 and recombinant human EPHA4 was shown in Figure 1, the EC50 for this effect is 0.4 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for EFNA3 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,350 Da
NCBI Official Full Name
ephrin-A3
NCBI Official Synonym Full Names
ephrin-A3
NCBI Official Symbol
EFNA3
NCBI Official Synonym Symbols
EFL2; EPLG3; LERK3; Ehk1-L
NCBI Protein Information
ephrin-A3; EFL-2; LERK-3; EHK1 ligand; ligand of eph-related kinase 3; eph-related receptor tyrosine kinase ligand 3
UniProt Protein Name
Ephrin-A3
UniProt Gene Name
EFNA3
UniProt Synonym Gene Names
EFL2; EPLG3; LERK3; EHK1-L; LERK-3
UniProt Entry Name
EFNA3_HUMAN
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Product Notes
The EFNA3 efna3 (Catalog #AAA161916) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ephrin A3 (EFNA3) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the EFNA3 efna3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Gly30~Ser2 38. It is sometimes possible for the material contained within the vial of "Ephrin A3 (EFNA3), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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