WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells using EFNA3 antibody.)
Rabbit EFNA3 Polyclonal Antibody | anti-EFNA3 antibody
EFNA3 Antibody
Gene Names
EFNA3; EFL2; EPLG3; LERK3; Ehk1-L
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Peptide affinity purification
Synonyms
EFNA3, Antibody; EFNA3 Antibody; EFL 2; EFL-2; EFL2; EFN A3; EFNA 3; Efna3; EFNA3_HUMAN; EHK1 L; EHK1 ligand; EHK1-L; EHK1L; EPH related receptor tyrosine kinase ligand 3; EPH-related receptor tyrosine kinase ligand 3; Ephrin-A3; EphrinA3; EPLG 3; EPLG3; LERK 3; LERK-3; LERK3; Ligand of eph related kinase 3; anti-EFNA3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
EFNA3 antibody detects endogenous levels of total EFNA3
Purity/Purification
Peptide affinity purification
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
238
Applicable Applications for anti-EFNA3 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Ephrin A3,corresponding to a region within C-terminal amino acids.
Conjugation
Unconjugated
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells using EFNA3 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells using EFNA3 antibody.)
IF (Immunofluorescence)
(AAA323910 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA323910 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-EFNA3 antibody
Function: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling (By similarity).
Subunit Structure: Interacts with EPHA8; activates EPHA8.
Similarity: Belongs to the ephrin family.
Subunit Structure: Interacts with EPHA8; activates EPHA8.
Similarity: Belongs to the ephrin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38 kD
NCBI Official Full Name
ephrin-A3
NCBI Official Synonym Full Names
ephrin A3
NCBI Official Symbol
EFNA3
NCBI Official Synonym Symbols
EFL2; EPLG3; LERK3; Ehk1-L
NCBI Protein Information
ephrin-A3
UniProt Protein Name
Ephrin-A3
UniProt Gene Name
EFNA3
UniProt Synonym Gene Names
EFL2; EPLG3; LERK3; EHK1-L; LERK-3
Similar Products
Product Notes
The EFNA3 efna3 (Catalog #AAA323910) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EFNA3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EFNA3 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the EFNA3 efna3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EFNA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.