SDS-PAGE
G6PD active protein
G6PD,1-491aa, E Coli, E Coli (Bioactivity Validated)
Gene Names
zwf; ECK1853; JW1841
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
G6PD; N/A; G6PD,1-491aa, E Coli, E Coli (Bioactivity Validated); Glucose-6-phosphate 1-dehydrogenase; zwf; Glucose-6-phosphate 1-dehydrogenase G6PD; G6PD1; G6pdx; Glucose 6 phosphate 1 dehydrogenase; MET19; POS10; Zwf1p.; G6PD active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
In 50 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E
Sequence Length
491
Applicable Applications for G6PD active protein
SDS-PAGE
Antigen Species
E Coli
Biological Activity
Specific activity is > 70 units/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for G6PD active protein
Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant G6PD protein was expressed in E Coli and purified by conventional chromatography techniques.
Product Categories/Family for G6PD active protein
References
Huang Y., et al. (2008) Mol Genet Metab. 93 (1):44-53.; Zimny A., et al. (2003) Pol Arch Med Wewn. 110 (5):1327-33;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55.7kDa (491 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
glucose-6-phosphate 1-dehydrogenase
NCBI Official Symbol
zwf
NCBI Official Synonym Symbols
ECK1853; JW1841
NCBI Protein Information
glucose-6-phosphate 1-dehydrogenase
UniProt Protein Name
Glucose-6-phosphate 1-dehydrogenase
UniProt Gene Name
zwf
UniProt Synonym Gene Names
EDF
Similar Products
Product Notes
The G6PD zwf (Catalog #AAA48520) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's G6PD can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the G6PD zwf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E. It is sometimes possible for the material contained within the vial of "G6PD, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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