Standard Curve (Sample)
Mouse Glucose 6 Phosphate Dehydrogenase ELISA Kit | G6PD elisa kit
Mouse Glucose 6 Phosphate Dehydrogenase ELISA Kit
Reactivity
Mouse
Synonyms
Glucose 6 Phosphate Dehydrogenase; N/A; Mouse Glucose 6 Phosphate Dehydrogenase ELISA Kit; G6PD elisa kit
Reactivity
Mouse
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
5.0-100?g/mL
Sensitivity
1.0?g/mL
Intended Uses
This G6PD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse G6PD. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for G6PD elisa kit
Principle of the Assay: G6PD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-G6PD antibody and an G6PD-HRP conjugate. The assay sample and buffer are incubated together with G6PD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the G6PD concentration since G6PD from samples and G6PD-HRP conjugate compete for the anti-G6PD antibody binding site. Since the number of sites is limited, as more sites are occupied by G6PD from the sample, fewer sites are left to bind G6PD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The G6PD concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Mouse G6PD (Catalog #AAA90177) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA90177 ELISA Kit recognizes Mouse G6PD. It is sometimes possible for the material contained within the vial of "Glucose 6 Phosphate Dehydrogenase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.