IL1B active protein

Recombinant Mouse IL1B

Cat. Num. AAA214313

• Gene Names: Il1b; Il-1b; IL-1beta
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IL1B; N/A; Recombinant Mouse IL1B; IL-1 beta; IL1b; Recombinant Mouse Interleukin-1 Beta (IL1B); IL1B active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.
• Sequence Length: 269

• Bioactivity: The ED(50) was determined by the dose-dependent stimulation of thymidine uptake by murine D10S cells is <= 2.0 pg/mL, corresponding to a specific activity of >= 5.0 x 10^8 units/mg.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Application Data

Application Data

Related Product Information for IL1B active protein

Monocytes are the main source of secreted IL-1. They express predominantly IL1-beta while human keratinocytes express large amounts of IL-1-alpha. Murine macrophages display a transition from IL-1-beta to IL-1-alpha production during maturation of monocytes into inflammatory macrophages. There are two functionally almost equivalent forms of IL-1, IL-1-alpha and IL-1-beta that are encoded by two different genes. IL-1-beta is the predominant form in humans while it is IL-1-alpha in mice. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL-1-beta but not the IL-1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T-cells and cells of mesenchymal origin. It binds both types of IL-1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.

NCBI and Uniprot Product Information

• NCBI GI #: 6680415
• NCBI GeneID: 16176
• NCBI Accession #: NP_032387.1
• NCBI GenBank Nucleotide #: NP_032387.1
• UniProt Accession #: P10749
• Molecular Weight: 17.0 kDa
• NCBI Official Full Name: interleukin-1 beta
• NCBI Official Synonym Full Names: interleukin 1 beta
• NCBI Official Symbol: Il1b
• NCBI Official Synonym Symbols: Il-1b; IL-1beta
• NCBI Protein Information: interleukin-1 beta
• UniProt Protein Name: Interleukin-1 beta
• UniProt Gene Name: Il1b
• UniProt Synonym Gene Names: IL-1 beta
• UniProt Entry Name: IL1B_MOUSE