Bioactivity
(Janus Kinase 1, also known as JAK1, is a member of the Janus kinase family, which plays a crucial role in various cellular processes, including signal transduction, immune response, and hematopoiesis. It is reported that the binding of JAK1 to IFNa2 plays an important role in immune response and antiviral defense. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human JAK1 and recombinant mouse IFNa2. Briefly, JAK1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IFNa2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-JAK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human JAK1 and recombinant mouse IFNa2 was shown in Figure 1, the EC50 for this effect is 0.1 ug/mL.)
Janus Kinase 1 (JAK1) Active Protein | JAK1 active protein
Active Janus Kinase 1 (JAK1)
Gene Names
JAK1; JTK3; JAK1A; JAK1B
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Synonyms
Janus Kinase 1 (JAK1); N/A; Active Janus Kinase 1 (JAK1); Janus Kinase 1; JAK1A; JAK1B; JTK3; Tyrosine-protein kinase JAK14; JAK1 active protein
Host
E Coli
Purity/Purification
Greater than 95% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
His596~Ile841
Applicable Applications for JAK1 active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
6.2
Research Area
Enzyme & Kinase
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Janus Kinase 1, also known as JAK1, is a member of the Janus kinase family, which plays a crucial role in various cellular processes, including signal transduction, immune response, and hematopoiesis. It is reported that the binding of JAK1 to IFNa2 plays an important role in immune response and antiviral defense. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human JAK1 and recombinant mouse IFNa2. Briefly, JAK1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IFNa2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-JAK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human JAK1 and recombinant mouse IFNa2 was shown in Figure 1, the EC50 for this effect is 0.1 ug/mL.)
Bioactivity
(Janus Kinase 1, also known as JAK1, is a member of the Janus kinase family, which plays a crucial role in various cellular processes, including signal transduction, immune response, and hematopoiesis. It is reported that the binding of JAK1 to IFNa2 plays an important role in immune response and antiviral defense. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human JAK1 and recombinant mouse IFNa2. Briefly, JAK1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IFNa2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-JAK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human JAK1 and recombinant mouse IFNa2 was shown in Figure 1, the EC50 for this effect is 0.1 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for JAK1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
133,277 Da
NCBI Official Full Name
tyrosine-protein kinase JAK1
NCBI Official Synonym Full Names
Janus kinase 1
NCBI Official Symbol
JAK1
NCBI Official Synonym Symbols
JTK3; JAK1A; JAK1B
NCBI Protein Information
tyrosine-protein kinase JAK1
UniProt Protein Name
Tyrosine-protein kinase JAK1
UniProt Gene Name
JAK1
UniProt Synonym Gene Names
JAK1A; JAK1B; JAK-1
UniProt Entry Name
JAK1_HUMAN
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Product Notes
The JAK1 jak1 (Catalog #AAA161890) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Janus Kinase 1 (JAK1) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the JAK1 jak1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: His596~Ile 841. It is sometimes possible for the material contained within the vial of "Janus Kinase 1 (JAK1), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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