Bioactivity
(A hallmark of most cancer cells is an altered metabolism involving a shift to aerobic glycolysis with lactate production coupled with a higher uptake of glucose as the main source of energy. Lactate dehydrogenase (LDH) is key to this shift by catalyzing the formation of lactate by reducing pyruvate with NADH, which also generates NAD() that is essential for the continuity of glycolysis. Inhibiting LDH activity has an anti-proliferative effect on cancer cells. It may reverse the resistance of tumor cells to conventional chemo- and radiotherapy. Recent research has renewed interest in LDH as an anticancer drug target. LDH enzymes have three homologous subunits LDHA, LDHB and LDHC. The activity of recombinant rat LDHC was measured by its ability to reduce pyruvate to lactate. The reaction was performed in 25 mM Tris, 100 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of rrLDHC (diluted by assay buffer) to 50 uL of substrate mixture 1.6 mM beta-NADH and 4 mM sodium pyruvate. The final well serves as a negative control with no rrLDHC, replaced with 50 ul assay buffer. Read at a wavelength of 340 nm in kinetic mode for 5 minutes. The specific activity of recombinant rat LDHC is > 880 pmol/min/ug.)
Lactate Dehydrogenase C (LDHC) Active Protein | LDHC active protein
Active Lactate Dehydrogenase C (LDHC)
Gene Names
Ldhc; Ldh3
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Synonyms
Lactate Dehydrogenase C (LDHC); N/A; Active Lactate Dehydrogenase C (LDHC); Lactate Dehydrogenase C; LDH-C; CT32; LDHX; LDH3; Cancer/Testis Antigen 32; LDH testis subunit4; LDHC active protein
Host
E Coli
Purity/Purification
Greater than 95% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Met1~Leu332
Applicable Applications for LDHC active protein
Activity Assay, Cell Culture
Species
Rat
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
8.1
Research Area
Enzyme & Kinase
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(A hallmark of most cancer cells is an altered metabolism involving a shift to aerobic glycolysis with lactate production coupled with a higher uptake of glucose as the main source of energy. Lactate dehydrogenase (LDH) is key to this shift by catalyzing the formation of lactate by reducing pyruvate with NADH, which also generates NAD() that is essential for the continuity of glycolysis. Inhibiting LDH activity has an anti-proliferative effect on cancer cells. It may reverse the resistance of tumor cells to conventional chemo- and radiotherapy. Recent research has renewed interest in LDH as an anticancer drug target. LDH enzymes have three homologous subunits LDHA, LDHB and LDHC. The activity of recombinant rat LDHC was measured by its ability to reduce pyruvate to lactate. The reaction was performed in 25 mM Tris, 100 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of rrLDHC (diluted by assay buffer) to 50 uL of substrate mixture 1.6 mM beta-NADH and 4 mM sodium pyruvate. The final well serves as a negative control with no rrLDHC, replaced with 50 ul assay buffer. Read at a wavelength of 340 nm in kinetic mode for 5 minutes. The specific activity of recombinant rat LDHC is > 880 pmol/min/ug.)
Bioactivity
(A hallmark of most cancer cells is an altered metabolism involving a shift to aerobic glycolysis with lactate production coupled with a higher uptake of glucose as the main source of energy. Lactate dehydrogenase (LDH) is key to this shift by catalyzing the formation of lactate by reducing pyruvate with NADH, which also generates NAD() that is essential for the continuity of glycolysis. Inhibiting LDH activity has an anti-proliferative effect on cancer cells. It may reverse the resistance of tumor cells to conventional chemo- and radiotherapy. Recent research has renewed interest in LDH as an anticancer drug target. LDH enzymes have three homologous subunits LDHA, LDHB and LDHC. The activity of recombinant rat LDHC was measured by its ability to reduce pyruvate to lactate. The reaction was performed in 25 mM Tris, 100 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of rrLDHC (diluted by assay buffer) to 50 uL of substrate mixture 1.6 mM beta-NADH and 4 mM sodium pyruvate. The final well serves as a negative control with no rrLDHC, replaced with 50 ul assay buffer. Read at a wavelength of 340 nm in kinetic mode for 5 minutes. The specific activity of recombinant rat LDHC is > 880 pmol/min/ug.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for LDHC active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
35,687 Da
NCBI Official Full Name
L-lactate dehydrogenase C chain
NCBI Official Synonym Full Names
lactate dehydrogenase C
NCBI Official Symbol
Ldhc
NCBI Official Synonym Symbols
Ldh3
NCBI Protein Information
L-lactate dehydrogenase C chain; LDH-C; LDH-X; LDH testis subunit; lactate dehydrogenase C variant 3; lactate dehydrogenase 3, C chain, sperm specific
UniProt Protein Name
L-lactate dehydrogenase C chain
UniProt Gene Name
Ldhc
UniProt Synonym Gene Names
Ldh-3; Ldh3; LDH-C
UniProt Entry Name
LDHC_RAT
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Product Notes
The LDHC ldhc (Catalog #AAA161921) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Lactate Dehydrogenase C (LDHC) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the LDHC ldhc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1~Leu33 2. It is sometimes possible for the material contained within the vial of "Lactate Dehydrogenase C (LDHC), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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