Nerve Growth Factor (NGF) Active Protein | NGF active protein

Active Nerve Growth Factor (NGF)

Cat. Num. AAA146613

• Gene Names: MMP8; HNC; CLG1; MMP-8; PMNL-CL
• Applications: Activity Assay, Cell Culture
• Purity: > 97%
• Synonyms: Nerve Growth Factor (NGF); N/A; Active Nerve Growth Factor (NGF); NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor; NGF active protein

• Host: E Coli
• Purity/Purification: > 97%
• Form/Format: Freeze-dried powder
• Sequence Length: 444

• Applicable Applications for NGF active protein: Activity Assay, Cell Culture
• Fragment: Glu19~Arg239
• Tag: N-terminal His Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Organism Species: Human
• Isoelectric Point: 10.1
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Application Data

Application Data

SDS-PAGE

Activity

(Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. As reported, when the pheochromocytoma cell line PC12 is exposed to nerve growth factor (NGF), the cells respond over a period of a week by ceasing cell division and extending neurites (Greene and Tischler, 1976). The cells were grown in Ham's F12K containing 5% fetal calf serum and 10% horse serum on  polylysine or collagen coated plates. When cells reached log phase growth , fresh medium was added together with 10ng/mL of NGF, then cells were observed by inverted microscope everyday.Cell division ceasing and differentiation of PC12 cells after incubation with NGF (10ng/mL) for 6 days was shown in Figure1.Control group which received no NGF displayed no neurite outgrowth and cells multiply rapidly.)

Product Categories/Family for NGF active protein

Active Protein

NCBI and Uniprot Product Information

• NCBI GI #: 751247063
• NCBI GeneID: 4317
• NCBI Accession #: NP_001291370.1
• NCBI GenBank Nucleotide #: NM_001304441.1
• UniProt Accession #: P22894; Q45F99
• Molecular Weight: 29.2kDa; 30/33kDa
• NCBI Official Full Name: neutrophil collagenase isoform 2
• NCBI Official Synonym Full Names: matrix metallopeptidase 8
• NCBI Official Symbol: MMP8
• NCBI Official Synonym Symbols: HNC; CLG1; MMP-8; PMNL-CL
• NCBI Protein Information: neutrophil collagenase
• UniProt Protein Name: Neutrophil collagenase
• UniProt Gene Name: MMP8
• UniProt Synonym Gene Names: CLG1; MMP-8; PMNL-CL