Bioactivity
(Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation. Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis. Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM). The activity assay of recombinant human NGAL was measured by its ability to bind Iron(III) dihydroxybenzoic acid Fe(DHBA)3. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in Lipocalin-2. The recombinant human NGAL was diluted to 100 ug/ml in assay buffer (50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5). 50 uL of the different concentrations of Fe(DHBA)3 and 50 uL of 100 ug/ml rmNGAL was loaded into the plate. Incubated at room temperature for 30 minutes, then read at excitation and emission wavelengths of 280 nM and 340 nM in endpoint mode. The result was shown in Figure 1, the recombinant human Lipocalin-2 can bind >8 uM of Fe(DHBA)3.)
Neutrophil gelatinase-associated lipocalin (NGAL) Active Protein | NGAL active protein
Active Neutrophil gelatinase-associated lipocalin (NGAL)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
Neutrophil gelatinase-associated lipocalin (NGAL); N/A; Active Neutrophil gelatinase-associated lipocalin (NGAL); Neutrophil gelatinase-associated lipocalin; Lipocalin-2; LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN24; NGAL active protein
Host
E Coli
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Gln21~Gly198
Applicable Applications for NGAL active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
9.2
Research Area
Infection immunity
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation. Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis. Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM). The activity assay of recombinant human NGAL was measured by its ability to bind Iron(III) dihydroxybenzoic acid Fe(DHBA)3. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in Lipocalin-2. The recombinant human NGAL was diluted to 100 ug/ml in assay buffer (50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5). 50 uL of the different concentrations of Fe(DHBA)3 and 50 uL of 100 ug/ml rmNGAL was loaded into the plate. Incubated at room temperature for 30 minutes, then read at excitation and emission wavelengths of 280 nM and 340 nM in endpoint mode. The result was shown in Figure 1, the recombinant human Lipocalin-2 can bind >8 uM of Fe(DHBA)3.)
Bioactivity
(Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation. Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis. Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM). The activity assay of recombinant human NGAL was measured by its ability to bind Iron(III) dihydroxybenzoic acid Fe(DHBA)3. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in Lipocalin-2. The recombinant human NGAL was diluted to 100 ug/ml in assay buffer (50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5). 50 uL of the different concentrations of Fe(DHBA)3 and 50 uL of 100 ug/ml rmNGAL was loaded into the plate. Incubated at room temperature for 30 minutes, then read at excitation and emission wavelengths of 280 nM and 340 nM in endpoint mode. The result was shown in Figure 1, the recombinant human Lipocalin-2 can bind >8 uM of Fe(DHBA)3.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for NGAL active protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
22,588 Da
NCBI Official Full Name
LCN2
UniProt Protein Name
Neutrophil gelatinase-associated lipocalin
UniProt Gene Name
LCN2
UniProt Synonym Gene Names
HNL; NGAL; NGAL
UniProt Entry Name
NGAL_HUMAN
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Product Notes
The NGAL lcn2 (Catalog #AAA161845) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Neutrophil gelatinase-associated lipocalin (NGAL) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the NGAL lcn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Gln21~Gly1 98. It is sometimes possible for the material contained within the vial of "Neutrophil gelatinase-associated lipocalin (NGAL), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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