SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
NQO1 active protein
Recombinant Human NQO1 Protein
Gene Names
NQO1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI
Applications
SDS-Page
Purity
>95% by SDS-PAGE
Synonyms
NQO1; N/A; Recombinant Human NQO1 Protein; NAD(P)H dehydrogenase quinone 1; DHQu; DIA4; DTD; NMOR1; NMORI; QR1; Azoreductase; Quinone reductase 1; DT-diaphorase; NAD(P)H dehydrogenase; quinone 1; Azoreductase.Menadione reductase; NQO 1; QR 1; Phylloquinone reductase; Cytochrome b 5 reductase; DIA 4; Diaphorase (NADH/NADPH); Diaphorase 4 Dioxin inducible 1; DT diaphorase; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase); NAD(P)H dehydrogenase quinone 1 NAD(P)H menadione oxidoreductase 1 dioxin inducible; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NMOR 1; NMOR I; NQO1 active protein
Host
E Coli
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid; In 20mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol 1mM DTT.
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MVGRRALIVL AHSERTSFNY AMKEAAAAAL KKKGWEVVES DLYAMNFNPI ISRKDITGKL KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIF QFPLQWFGVP AILKGWFERV FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQG IHGDMNVILW PIQSGILHFC GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETP LYFAPSSLFD LNFQAGFLMK KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK
Sequence Length
274
Applicable Applications for NQO1 active protein
SDS-PAGE, Enzyme Activity
Endotoxin
<1 EU per 1ug of protein (determined by LAL method).
Domain
1-274aa
Tag
His-Tag
Biological Activity
Specific activity is > 1,000 pmol/min/ug and is defined as the amount of enzyme that converts 1 pmole of resazurin to resorufin per minute at pH 7.5 at 25C.
Preparation and Storage
Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for NQO1 active protein
NQO1 is a member of the NAD (P) H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This protein apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinines thus preventing the formation of reactive oxygen species. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD). Recombinant human NQO1, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for NQO1 active protein
References
Jaiswal AK., et al. (1988) J Biol Chem. 263(27):13572-8. Traver RD., et al. (1992) Cancer Res. 52(4):797-802.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
33.0 kDa (294aa), confirmed by MALDI-TOF.
NCBI Official Full Name
NAD(P)H dehydrogenase
NCBI Official Synonym Full Names
NAD(P)H quinone dehydrogenase 1
NCBI Official Symbol
NQO1
NCBI Official Synonym Symbols
DTD; QR1; DHQU; DIA4; NMOR1; NMORI
NCBI Protein Information
NAD(P)H dehydrogenase [quinone] 1
UniProt Protein Name
NAD(P)H dehydrogenase [quinone] 1
UniProt Gene Name
NQO1
UniProt Synonym Gene Names
DIA4; NMOR1; DTD; QR1
UniProt Entry Name
NQO1_HUMAN
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Product Notes
The NQO1 nqo1 (Catalog #AAA11735) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NQO1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Enzyme Activity. Researchers should empirically determine the suitability of the NQO1 nqo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MVGRRALIVL AHSERTSFNY AMKEAAAAAL KKKGWEVVES DLYAMNFNPI ISRKDITGKL KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIF QFPLQWFGVP AILKGWFERV FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQG IHGDMNVILW PIQSGILHFC GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETP LYFAPSSLFD LNFQAGFLMK KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK. It is sometimes possible for the material contained within the vial of "NQO1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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