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product-image-AAA153141_BIOACTIVITY13.jpg Bioactivity (PNGase F (Peptide-N-glycosidase F) is a kind of enzymes for the deglycosylation of glycoproteins. The enzyme releases asparagine-linked oligosaccharides from glycoproteins and glycopeptides by hydrolyzing the amide of the asparagine (Asn) side chain. Thus)

Peptide-N4-N-Acetyl-Beta-D-Glucosaminyl Asparagine Amidase F (PNGaseF) Active Protein | PNGaseF active protein

Active Peptide-N4-N-Acetyl-Beta-D-Glucosaminyl Asparagine Amidase F (PNGaseF)

Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
Peptide-N4-N-Acetyl-Beta-D-Glucosaminyl Asparagine Amidase F (PNGaseF); N/A; Active Peptide-N4-N-Acetyl-Beta-D-Glucosaminyl Asparagine Amidase F (PNGaseF); Peptide-N4-N-Acetyl-Beta-D-Glucosaminyl Asparagine Amidase F; PNGaseF active protein (prokaryotic), General species; Ngl; PNG; PNGase F; Glycopeptide N-glycosidase; N-glycanase; PNGaseF active protein
Ordering
Host
E coli
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Freeze-dried powder
Sequence Positions
Ala41~Asn354
Applicable Applications for PNGaseF active protein
Activity Assay, Cell Culture
Expression System
Prokaryotic expression
Species
General species
Tag
N-terminal His
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
8
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Stable for 12 months if correctly stored.

Bioactivity

(PNGase F (Peptide-N-glycosidase F) is a kind of enzymes for the deglycosylation of glycoproteins. The enzyme releases asparagine-linked oligosaccharides from glycoproteins and glycopeptides by hydrolyzing the amide of the asparagine (Asn) side chain. Thus)

product-image-AAA153141_BIOACTIVITY13.jpg Bioactivity (PNGase F (Peptide-N-glycosidase F) is a kind of enzymes for the deglycosylation of glycoproteins. The enzyme releases asparagine-linked oligosaccharides from glycoproteins and glycopeptides by hydrolyzing the amide of the asparagine (Asn) side chain. Thus)

SDS-PAGE

(Figure. SDS-PAGE)

product-image-AAA153141_SDS_PAGE15.jpg SDS-PAGE (Figure. SDS-PAGE)
Product Categories/Family for PNGaseF active protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,032 Da
NCBI Official Full Name
MULTISPECIES: peptide-N(4)-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F
UniProt Protein Name
Peptide-N(4)-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F
UniProt Gene Name
ngl
UniProt Synonym Gene Names
png; PNGase F

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Product Notes

The PNGaseF ngl (Catalog #AAA153141) is an Active Protein produced from E coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Ala41~Asn354. AAA Biotech's Peptide-N4-N-Acetyl-Beta-D-Glucosaminyl Asparagine Amidase F (PNGaseF) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the PNGaseF ngl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peptide-N4-N-Acetyl-Beta-D-Glucosaminyl Asparagine Amidase F (PNGaseF), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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