Bioactivity
(S100 Calcium Binding Protein A7 (S100A7), a member of the S100 family of vertebrate proteins, is an EF-hand type calcium binding protein localized in epithelial cells. S100A7 regulates cell proliferation and differentiation. An S100A7 overexpression may occur in response to inflammatory stimuli, such in psoriasis, besides S100A7 expression is upregulated in several cancers including skin, breast, lung, head, neck, cervix, bladder and gastric cancer. S100 Calcium Binding Protein A12 (S100A12) has been identified as an interactor of S100A7, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human S100A7 and recombinant pig S100A12. Briefly, S100A7 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to S100A12-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100A7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human S100A7 and recombinant pig S100A12 was shown in Figure 1, the EC50 for this effect is 0.12 ug/mL.)
S100 Calcium Binding Protein A7 (S100A7) Active Protein | S100A7 active protein
Active S100 Calcium Binding Protein A7 (S100A7)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
S100 Calcium Binding Protein A7 (S100A7); N/A; Active S100 Calcium Binding Protein A7 (S100A7); S100 Calcium Binding Protein A7; S100-A7; PSOR1; S100A7c; Psoriasin 14; S100A7 active protein
Host
E Coli
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Met1~Gln101
Applicable Applications for S100A7 active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
6.7
Research Area
Tumor immunity, Infection immunity
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(S100 Calcium Binding Protein A7 (S100A7), a member of the S100 family of vertebrate proteins, is an EF-hand type calcium binding protein localized in epithelial cells. S100A7 regulates cell proliferation and differentiation. An S100A7 overexpression may occur in response to inflammatory stimuli, such in psoriasis, besides S100A7 expression is upregulated in several cancers including skin, breast, lung, head, neck, cervix, bladder and gastric cancer. S100 Calcium Binding Protein A12 (S100A12) has been identified as an interactor of S100A7, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human S100A7 and recombinant pig S100A12. Briefly, S100A7 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to S100A12-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100A7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human S100A7 and recombinant pig S100A12 was shown in Figure 1, the EC50 for this effect is 0.12 ug/mL.)
Bioactivity
(S100 Calcium Binding Protein A7 (S100A7), a member of the S100 family of vertebrate proteins, is an EF-hand type calcium binding protein localized in epithelial cells. S100A7 regulates cell proliferation and differentiation. An S100A7 overexpression may occur in response to inflammatory stimuli, such in psoriasis, besides S100A7 expression is upregulated in several cancers including skin, breast, lung, head, neck, cervix, bladder and gastric cancer. S100 Calcium Binding Protein A12 (S100A12) has been identified as an interactor of S100A7, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human S100A7 and recombinant pig S100A12. Briefly, S100A7 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to S100A12-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100A7 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human S100A7 and recombinant pig S100A12 was shown in Figure 1, the EC50 for this effect is 0.12 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for S100A7 active protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The S100A7 s100a7 (Catalog #AAA161876) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's S100 Calcium Binding Protein A7 (S100A7) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the S100A7 s100a7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1~Gln10 1. It is sometimes possible for the material contained within the vial of "S100 Calcium Binding Protein A7 (S100A7), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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