Application Data
SHH active protein
Recombinant Human SHH
Gene Names
SHH; TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5
Purity
>95% as determined by SDS-PAGE
Synonyms
SHH; N/A; Recombinant Human SHH; HHG-1; Recombinant Human Sonic Hedgehog (SHH); SHH active protein
Host
E Coli
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from a 0.2 um filtered solution in Phosphate buffer and NaCl
Sequence Length
462
Bioactivity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1--0.4 ug/mL.
Endotoxin Level
<1.0 EU/ug of recombinant protein as determined by the LAL method
Reconstitution
A quick spin of the vial followed by reconstitution at 100 ug/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Preparation and Storage
The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for SHH active protein
Highly conserved proteins, members of the Hedgehog family are widely represented throughout the animal kingdom. The three known mammalian Hedgehog proteins; Sonic (SHH), Desert (DHH) and Indian (IHH) hedgehog share a high degree of sequence and structural identity. SHH is specifically known to be expressed in fetal intestine, liver, lung and kidney. Recombinant Human SHH represents the N-product (N-terminal domain with Cys24 substituted by an Ile-Val-Ile sequence) which retains all the known signalling capabilities.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
20.1 kDa
NCBI Official Full Name
Sonic hedgehog protein
NCBI Official Synonym Full Names
sonic hedgehog signaling molecule
NCBI Official Symbol
SHH
NCBI Official Synonym Symbols
TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5
NCBI Protein Information
sonic hedgehog protein
UniProt Protein Name
Sonic hedgehog protein
UniProt Gene Name
SHH
UniProt Synonym Gene Names
SHH
UniProt Entry Name
SHH_HUMAN
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Product Notes
The SHH shh (Catalog #AAA214271) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SHH, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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