SDS-PAGE
SurA active protein
SurA, 21-428aa, E Coli, His tag, E Coli (Bioactivity Validated)
Gene Names
surA; ECK0054; JW0052
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
SurA; N/A; SurA, 21-428aa, E Coli, His tag, E Coli (Bioactivity Validated); Chaperone surA; PPIase surA; Rotamase surA; Survival protein A; SurA active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MAPQVVDKVA AVVNNGVVLE SDVDGLMQSV KLNAAQARQQ LPDDATLRHQ IMERLIMDQI ILQMGQKMGV KISDEQLDQA IANIAKQNNM TLDQMRSRLA YDGLNYNTYR NQIRKEMIIS EVRNNEVRRR ITILPQEVES LAQQVGNQND ASTELNLSHI LIPLPENPTS DQVNEAESQA RAIVDQARNG ADFGKLAIAH SADQQALNGG QMGWGRIQEL PGIFAQALST AKKGDIVGPI RSGVGFHILK VNDLRGESKN ISVTEVHARH ILLKPSPIMT DEQARVKLEQ IAADIKSGKT TFAAAAKEFS QDPGSANQGG DLGWATPDIF DPAFRDALTR LNKGQMSAPV HSSFGWHLIE LLDTRNVDKT DAAQKDRAYR MLMNRKFSEE AASWMQEQRA SAYVKILSN
Sequence Length
428
Applicable Applications for SurA active protein
SDS-PAGE
Antigen Species
E Coli
Tag Information
His-tag
Biological Activity
Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for SurA active protein
SurA is a periplasmic peptidyl-prolyl isomerase (PPIase) and chaperone of Escherichia coli and other Gram-negative bacteria. This protein is a major factor in the biogenesis of beta-barrel outer membrane proteins (OMPs) and plays an integral role in cell envelope homeostasis and cell envelope functions. It is essential for the survival of E Coli in stationary phase and required for pilus biogenesis. Recombinant E Coli SurA protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for SurA active protein
References
Virlogeux-Payant I., et al (2009) Microbiology. 155 (Pt 5):1613-22; Kolter R., et al. (1996) J Bacteriol. 178 (6):1770-3.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47.3 kDa (429aa), confirmed by MALDI-TOF
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase (PPIase)
NCBI Official Symbol
surA
NCBI Official Synonym Symbols
ECK0054; JW0052
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase (PPIase)
UniProt Protein Name
Chaperone SurA
UniProt Gene Name
surA
UniProt Synonym Gene Names
PPIase SurA
Similar Products
Product Notes
The SurA sura (Catalog #AAA48603) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's SurA can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SurA sura for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MAPQVVDKVA AVVNNGVVLE SDVDGLMQSV KLNAAQARQQ LPDDATLRHQ IMERLIMDQI ILQMGQKMGV KISDEQLDQA IANIAKQNNM TLDQMRSRLA YDGLNYNTYR NQIRKEMIIS EVRNNEVRRR ITILPQEVES LAQQVGNQND ASTELNLSHI LIPLPENPTS DQVNEAESQA RAIVDQARNG ADFGKLAIAH SADQQALNGG QMGWGRIQEL PGIFAQALST AKKGDIVGPI RSGVGFHILK VNDLRGESKN ISVTEVHARH ILLKPSPIMT DEQARVKLEQ IAADIKSGKT TFAAAAKEFS QDPGSANQGG DLGWATPDIF DPAFRDALTR LNKGQMSAPV HSSFGWHLIE LLDTRNVDKT DAAQKDRAYR MLMNRKFSEE AASWMQEQRA SAYVKILSN. It is sometimes possible for the material contained within the vial of "SurA, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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