SDS-PAGE
(SDS-PAGE analysis: Lane 1: MW marker, Lane 2: 1ug TRAIL (soluble) (human), (recombinant) (His-tag) (Prod. No. BML-SE721).)
TRAIL active protein
TRAIL (soluble) (human), (recombinant) (His-tag)
Gene Names
TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L
Purity
>=95% (SDS-PAGE)Purified by multi-step chromatography
Synonyms
TRAIL; N/A; TRAIL (soluble) (human), (recombinant) (His-tag); TRAIL active protein
Host
E Coli
Specificity
Binds to human and mouse TRAIL receptors and human osteoprotegerin (OPG)
Purity/Purification
>=95% (SDS-PAGE)
Purified by multi-step chromatography
Purified by multi-step chromatography
Form/Format
Liquid. In PBS, containing 10% glycerol and 0.1mM DTT
Source Note
Human TRAIL (aa 114-281) is fused to a His-tag
Endotoxin
<0.1EU/ug TRAIL, determined using chromogenic Limulus amebocyte lysate (LAL) test
Biological Activity
Induces apoptosis in U937 cells at 1ug/ml, within 5 hours. At 0.5-2.0ug/ml, induces apoptosis in various thyroid carcinoma lines within 18 hours. User must determine optimal concentrations and time course for induction. Please note that this protein does not require a cross-linking enhancer to exhibit this potent activity
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Ships on Dry Ice
When stored at -80°C, this protein is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the protein, followed by refreezing, could lead to loss of activity.
After initial defrost, aliquot product into individual tubes and refreeze at -70°C. Avoid freeze/thaw cycles.
When stored at -80°C, this protein is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the protein, followed by refreezing, could lead to loss of activity.
After initial defrost, aliquot product into individual tubes and refreeze at -70°C. Avoid freeze/thaw cycles.
SDS-PAGE
(SDS-PAGE analysis: Lane 1: MW marker, Lane 2: 1ug TRAIL (soluble) (human), (recombinant) (His-tag) (Prod. No. BML-SE721).)
SDS-PAGE
(SDS-PAGE analysis: Lane 1: MW marker, Lane 2: 1ug TRAIL (soluble) (human), (recombinant) (His-tag) (Prod. No. BML-SE721).)
WB (Western Blot)
(Western Blot Analysis: Lane 1: MW marker, Lane 2: 50 ng of TRAIL (soluble) (human), (recombinant) (His-tag) probed with Anti-6X His tag® antibody.)
WB (Western Blot)
(Western Blot Analysis: Lane 1: MW marker, Lane 2: 50 ng of TRAIL (soluble) (human), (recombinant) (His-tag) probed with Anti-6X His tag® antibody.)
Related Product Information for TRAIL active protein
TRAIL, also called Apo2L, is a member of the tumor necrosis factor family (TNF). In contrast to other family members such as TNFa/TNFR1 and FasL/Fas, TRAIL and its receptors DR4 and DR5 are widely expressed in many human tissues. TRAIL activates rapid apoptosis in many transformed cell lines but not in normal tissues, even though TRAIL and its receptors DR4 and DR5 are expressed on both cell types. A glycophospholipid-anchored cell surface protein designated decoy receptor 1 (DcR1 or TRID) acts as a decoy to inhibit TRAIL signaling. Thus, TRAIL and its signaling pathways may have direct applications in selective tumor cell death and cancer research.
Product Categories/Family for TRAIL active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~23kDa
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 10 isoform 2
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 10
NCBI Official Symbol
TNFSF10
NCBI Official Synonym Symbols
TL2; APO2L; CD253; TRAIL; Apo-2L
NCBI Protein Information
tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor apoptosis-
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 10
UniProt Gene Name
TNFSF10
UniProt Synonym Gene Names
APO2L; TRAIL; Apo-2L
UniProt Entry Name
TNF10_HUMAN
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Product Notes
The TRAIL tnfsf10 (Catalog #AAA77052) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TRAIL, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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