TRANCE/RANK L/TNFSF11 active protein

Recombinant TRANCE/RANK L/TNFSF11

Cat. Num. AAA48324

• Gene Names: Tnfsf11; ODF; OPGL; RANKL; Ly109l; Trance
• Applications: SDS-Page
• Purity: > 90% by SDS - PAGE
• Synonyms: TRANCE/RANK L/TNFSF11; N/A; Recombinant TRANCE/RANK L/TNFSF11; CD254; hRANKL2; ODF; OPGL; OPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANKL; Receptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF related activation induced cytokine; TNF-related activation-induced cytokine; TNF11_HUMAN; TNFSF 11; Tnfsf11; TRANCE; Tumor necrosis factor (ligand) superfamily member 11; Tumor necrosis factor ligand superfamily member 11; soluble form; TRANCE/RANK L/TNFSF11 active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS - PAGE
• Form/Format: Liquid. In Tris-Hcl buffer(pH8.5) containing 0.1M NaCl
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 158-316aa
• Sequence: MKPEAQPFAH LTINAASIPS GSHKVTLSSW YHDRGWAKIS NMTLSNGKLR VNQDGFYYLY ANICFRHHET SGSVPTDYLQ LMVYVVKTSI KIPSSHNLMK GGSTKNWSGN SEFHFYSINV GGFFKLRAGE EISIQVSNPS LLDPDQDATY FGAFKVQDID
• Sequence Length: 316

• Applicable Applications for TRANCE/RANK L/TNFSF11 active protein: Bioactivity, SDS-PAGE
• Tag: Non-Tagged
• Biological Activity: Measured in a osteoclast differentiation assay using RAW 264.7 mouse monocyte/macrophage cells. The ED50 range <= 2 ng/ml.
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Mouse TRANCE/RANK L/TNFSF11 stimulates osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cells. The ED50 range )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for TRANCE/RANK L/TNFSF11 active protein

Tnfsf11, also known as tumor necrosis factor ligand superfamily member 11, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. Tnfsf11 also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. Tnfsf11 is important in bone metabolism. This natural and necessary surface-bound molecule (also known as CD254) found on osteoblasts serves to activate osteoclasts, which are the cells involved in bone resorption. Recombinant Mouse Tnfsf11 protein was expressed in E. coli and purified by using conventional chromatography.

Product Categories/Family for TRANCE/RANK L/TNFSF11 active protein

Hormones & Cytokines

References

Lam J., et al. (2001). J. Clin. Invest. 108(7):971-9.;;Ito S., et al. (2002). J. Biol. Chem. 277(8):6631-6.

NCBI and Uniprot Product Information

• NCBI GI #: 114842415
• NCBI GeneID: 21943
• NCBI Accession #: NP_035743.2
• NCBI GenBank Nucleotide #: NP_035743.2
• UniProt Accession #: O35235
• Molecular Weight: 17.9 kDa (160aa), confirmed by MALDI-TOF
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 11
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 11
• NCBI Official Symbol: Tnfsf11
• NCBI Official Synonym Symbols: ODF; OPGL; RANKL; Ly109l; Trance
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• UniProt Gene Name: Tnfsf11
• UniProt Synonym Gene Names: Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE
• UniProt Entry Name: TNF11_MOUSE