Biological Activity
(Human TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.)
Tumor Necrosis Factor-alpha Active Protein | TNF active protein
Tumor Necrosis Factor-alpha, 77-233 aa, Human, E Coli (Bioactivity Validated)
Gene Names
TNF; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
Tumor Necrosis Factor-alpha; N/A; Tumor Necrosis Factor-alpha, 77-233 aa, Human, E Coli (Bioactivity Validated); Tumor necrosis factor alpha; TNFA; TNFSF2; DIF (differentiation inducing factor); CACHECTIN; DIF; TNF-alpha; TNF; tumor necrosis factor (TNF superfamily; member 2); TNF superfamily member 2,; TNF active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4)
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Applicable Applications for TNF active protein
SDS-PAGE, Bioactivity
Domain
77-233aa
Tag
Non-tagged
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Biological Activity
Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 range < 0.2 ng/mL.
The ED50 range < 0.2 ng/mL.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Can be stored at 2°C to 8°C for one week. For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
Avoid repeated freezing and thawing cycles.
Biological Activity
(Human TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.)
Biological Activity
(Human TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for TNF active protein
Tumor necrosis factor alpha (TNF-alpha), also called cachectin, consists of 157 amino acids. TNF-alpha is a 17.5 kD factor produced by neutrophils, CD4+ T cells, macrophage, NK cells, LAK cells, astrocytes endothelial cells, and smooth muscle cells. TNF-alpha is cytolytic and plays an important role in immune regulation including hemorrhagic tumor necrosis/cytotoxicity and inflammation, and in regulation of antiviral and immune proliferative and activation responses. The active form of this protein is a trimer. Recombinant human TNF-alpha was expressed in E. coli and purified by using conventional chromatography techniques. Additional amino acid, methionine, was attached at N-terminus of the protein.
Product Categories/Family for TNF active protein
References
Chicoine M.R., et al. (1997), J. Neurooncol. 35: 249-57; Ruddle N.H., et al. (1992), Curr. Opin. Immunol. 4: 327-32; Wingfield P., et al. (1987), FEBS Lett. 211:179-184;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17.5 kDa (158 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
tumor necrosis factor
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
NCBI Protein Information
tumor necrosis factor
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
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Product Notes
The TNF tnf (Catalog #AAA11742) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Tumor Necrosis Factor-alpha can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Bioactivity. Researchers should empirically determine the suitability of the TNF tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor-alpha, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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