Porcine Tumor Necrosis Factor Alpha ELISA Kit | TNF-alpha elisa kit

Porcine Tumor Necrosis Factor Alpha ELISA Kit

Cat. Num. AAA17368

• Reactivity: Porcine
• Synonyms: Tumor Necrosis Factor Alpha; N/A; Porcine Tumor Necrosis Factor Alpha ELISA Kit; NF? ELISA Kit, Tumor Necrosis Factor Alpha ELISA Kit, TNF-? ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit; TNF-alpha elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of TNF-alpha. No significant cross-reactivity or interference between TNF-alpha and analogues was observed.
• Sequence Length: 250
• Assay Type: Sandwich
• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml

• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for TNF-alpha elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 83921651
• NCBI GeneID: 654303
• NCBI Accession #: NP_001033074.1
• NCBI GenBank Nucleotide #: NM_001037985.1
• NCBI Official Full Name: tumor necrosis factor alpha
• NCBI Official Symbol: tnfa
• NCBI Protein Information: tumor necrosis factor alpha