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WB (Western Blot)
(Sample: Recombinant TNFRSF12A, Human; Antibody: Rabbit Anti-Human TNFRSF12A Ab)
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) Active Protein | TNFRSF12A active protein
Active Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A)
Gene Names
TNFRSF12A; FN14; CD266; TWEAKR
Applications
Activity Assay, Cell Culture
Purity
>85%
Synonyms
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A); N/A; Active Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A); CD266; TNFRSF12-A; FN14; TWEAKR; Tumour Necrosis Factor Related Weak Inducer Of Apoptosis; Fibroblast growth factor-inducible immediate-early response protein 14; TNFRSF12A active protein
Host
E. coli
Purity/Purification
>85%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl and 5% trehalose.
Concentration
Original Concentration: 50ug/mL (varies by lot)
Applicable Applications for TNFRSF12A active protein
Activity Assay, Cell Culture
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression.
Residues
Ser24~Ala126
Tags
Two N-terminal Tags, His-tag and SUMO-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
6.7
Usage
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Sample: Recombinant TNFRSF12A, Human; Antibody: Rabbit Anti-Human TNFRSF12A Ab)
WB (Western Blot)
(Sample: Recombinant TNFRSF12A, Human; Antibody: Rabbit Anti-Human TNFRSF12A Ab)
SDS_PAGE
(SDS-PAGE Sample: Active recombinant TNFRSF12A, Human)
SDS_PAGE
(SDS-PAGE Sample: Active recombinant TNFRSF12A, Human)
Gene Sequencing
(Gene Sequencing (extract))
Gene Sequencing
(Gene Sequencing (extract))
Gene Sequence
Gene Sequence
Activity
(TNFRSF12A (Tumor necrosis factor receptor superfamily member 12A) belongs to the Tumor necrosis factor receptor superfamily. TNFRSF12A is thought to induce apoptosis weakly in some cell types and promotes angiogenesis and the proliferation of endothelial cells. A binding ELISA assay was conducted to detect the association of TNFRSF12A with TNFa. Briefly, TNFRSF12A were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TNFRSF12A were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF12A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF12A and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.)
Activity
(TNFRSF12A (Tumor necrosis factor receptor superfamily member 12A) belongs to the Tumor necrosis factor receptor superfamily. TNFRSF12A is thought to induce apoptosis weakly in some cell types and promotes angiogenesis and the proliferation of endothelial cells. A binding ELISA assay was conducted to detect the association of TNFRSF12A with TNFa. Briefly, TNFRSF12A were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TNFRSF12A were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF12A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF12A and TNFa was shown in Figure 1, and this effect was in a dose dependent manner.)
Product Categories/Family for TNFRSF12A active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 19.8kDa
Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 12A
NCBI Official Synonym Full Names
TNF receptor superfamily member 12A
NCBI Official Symbol
TNFRSF12A
NCBI Official Synonym Symbols
FN14; CD266; TWEAKR
NCBI Protein Information
tumor necrosis factor receptor superfamily member 12A
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 12A
UniProt Gene Name
TNFRSF12A
UniProt Synonym Gene Names
FN14; FGF-inducible 14; TweakR
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Product Notes
The TNFRSF12A tnfrsf12a (Catalog #AAA148180) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the TNFRSF12A tnfrsf12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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