TWEAKR Active Protein | TNFRSF12A active protein

TWEAKR Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA257882

• Gene Names: TNFRSF12A; FN14; CD266; TWEAKR
• Purity: >95% as determined by SDS-PAGE
• Synonyms: TWEAKR; N/A; TWEAKR Protein, Human, Recombinant (hFc Tag); Human TNFRSF12A/FN14/TWEAKR Protein (Fc Tag); CD266 Protein, Human; FN14 Protein, Human; TWEAKR Protein, Human; tumor necrosis factor receptor superfamily member 12A; TNFRSF12A active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Glu28-Trp79

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human TNFRSF12A isoform 1 (Q9NP84-1) extracellular domain (Glu 28-Trp 79) was fused with the Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Immobilized Cynomolgus mFc-TNFSF12 at 10ug/ml (100uL/well) can bind human Fc-TNFRSF12A. The ED50 of human Fc-TNFRSF12A is 0.07-0.15ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Immobilized Cynomolgus mFc-TNFSF12 at 10 ug/ml (100 ul/well) can bind human Fc-TNFRSF12A, The ED50 of human Fc-TNFRSF12A is 0.07-0.15 ug/ml.)

SDS-PAGE

Related Product Information for TNFRSF12A active protein

Background: Fn14 (tumor necrosis factor receptor superfamily, member 12A), also known as TNFRSF12A, is the receptor for TNFSF12/TWEAK. Fn14 shares 82% amino acid identity with the mouse sequence. It contains a signal peptide, an extracellular domain, a membrane-anchoring domain, and a cytoplasmic domain. In response to FGF1, calf serum, or phorbol ester stimulation of human quiescent fibroblasts in vitro, the level of Fn14 is increased. A 1.2-kb FN14 transcript was expressed at high levels in heart, placenta, and kidney, at intermediate levels in lung, skeletal muscle, and pancreas, and at low levels in brain and liver. Also, elevated FN14 expression was found in human liver cancer cell lines and hepatocellular carcinoma specimens. Expression of mouse Fn14 was upregulated in hepatocellular carcinoma nodules that develop in 2 different transgenic mouse models of hepatocarcinogenesis. TNFRSF12A is the weak inducer of apoptosis in some cell types. It promotes angiogenesis and the proliferation of endothelial cells. TNFRSF12A may modulate cellular adhesion to matrix proteins.

References

Burkly LC, et al. (2011) The TWEAK/Fn14 pathway in tissue remodeling: for better or for worse. Adv Exp Med Biol. 691:305-22.Huang M, et al. (2011) Overexpression of Fn14 promotes androgen-independent prostate cancer progression through MMP-9 and correlates with poor treatment outcome. Carcinogenesis. 32(11):1589-96.Dharmapatni AA, et al. (2011) TWEAK and Fn14 expression in the pathogenesis of joint inflammation and bone erosion in rheumatoid arthritis. Arthritis Res Ther. 13(2):R51.

NCBI and Uniprot Product Information

• NCBI GI #: 7706186
• NCBI GeneID: 51330
• NCBI Accession #: NP_057723.1
• NCBI GenBank Nucleotide #: NM_016639.2
• UniProt Accession #: Q9NP84; Q9HCS0; D3DUA6
• Molecular Weight: 10,191 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 12A
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 12A
• NCBI Official Symbol: TNFRSF12A
• NCBI Official Synonym Symbols: FN14; CD266; TWEAKR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 12A; FGF-inducible 14; fibroblast growth factor-inducible immediate-early response protein 14; tweak-receptor; type I transmembrane protein Fn14
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 12A
• UniProt Gene Name: TNFRSF12A
• UniProt Synonym Gene Names: FN14; FGF-inducible 14; TweakR
• UniProt Entry Name: TNR12_HUMAN