Melanin and Catecholamine Synthesis Pathways
Tyrosinase Active Protein | TYR active protein
Tyrosinase (human), (recombinant) (His-tag)
Gene Names
TYR; CMM8; OCA1A; OCAIA; SHEP3
Purity
>=85% (SDS-PAGE)Purified by multi-step chromatography
Synonyms
Tyrosinase; N/A; Tyrosinase (human), (recombinant) (His-tag); TYR active protein
Host
E Coli
Purity/Purification
>=85% (SDS-PAGE)
Purified by multi-step chromatography
Purified by multi-step chromatography
Form/Format
Liquid. In 50mM TRIS/HCl, pH8.0, 1M NaCl, 0.05% Empigen BB, and 30% glycerol
Activity
One microgram will produce greater than 90pmol of adduct at 37 degree C using 0.5mM L-Tyrosine as substrate in 50mM potassium phosphate, pH7.3, 2% dimethylformamide, 5mM MBTH (3-methyl-2-benzothiazolinone hydrazone) and monitoring formation of dopachrome-MBTH adduct at 484nm (epsilon484=22,300 M-1cm-1). Note: tyrosinases, in particular mammalian tyrosinase, are inherently inefficient enzymes, with typical changes in OD only in the deciOD range, although the reaction reaches maximal velocity quickly (typically within two minutes). In addition, its substrates and MBTH are very unstable; make stocks fresh and keep sealed in the dark until use. Lifetime and rate of enzymatic activity can be increased by the addition of CuCl to the assay buffer, but this will increase background and may complicate other redox reactions in the assay
Preparation and Storage
For long term storage, store at -80 degree C. Avoid freeze/thaw cycles
Shipping: Shipped on Dry Ice
Shipping: Shipped on Dry Ice
Melanin and Catecholamine Synthesis Pathways
Melanin and Catecholamine Synthesis Pathways
Tyrosinase Activity
(Formation of dopachrome-MBTH adduct monitoring at 484nm in a 96-well half area microwell plate with a range of enzyme loads. Maximum signal plateau was reached rapidly at 37 degree C, preventing an accurate time-dependent measurement.)
Tyrosinase Activity
(Formation of dopachrome-MBTH adduct monitoring at 484nm in a 96-well half area microwell plate with a range of enzyme loads. Maximum signal plateau was reached rapidly at 37 degree C, preventing an accurate time-dependent measurement.)
SDS-PAGE
(Lane 1: MW Marker, Lane 2: 2 ug Tyrosinase.)
SDS-PAGE
(Lane 1: MW Marker, Lane 2: 2 ug Tyrosinase.)
Related Product Information for TYR active protein
Tyrosinase is a copper-containing enzyme that catalyzes four steps in the melanin and catecholamine synthesis pathways. Like tyrosine hydroxylase (EC 1.13.16.2), it can catalyze the formation of L-DOPA in the brain and nervous system. It oxidizes monophenols, but like catecholoxidase (EC 1.10.3.1), it can also oxidize o-diphenols to o-quinones. It is involved in cancer, Parkinson's, pigmentation diseases, Alzheimer's and obesity. Much of the research and inhibitor screening has used mushroom tyrosinase. However, mushroom tyrosinase interacts very differently with substrates and inhibitors compared to the human enzyme. In addition, commercially available mushroom tyrosinase is not always of good purity. Recombinant versions of human tyrosinase amino acids 1-345 or 1-378, also available commercially, are not functional due the lack of critical catalytic and structural residues.
Product Categories/Family for TYR active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~52kDa (SDS-PAGE)
NCBI Official Full Name
tyrosinase
NCBI Official Synonym Full Names
tyrosinase
NCBI Official Symbol
TYR
NCBI Official Synonym Symbols
CMM8; OCA1A; OCAIA; SHEP3
NCBI Protein Information
tyrosinase; LB24-AB; SK29-AB; monophenol monooxygenase; oculocutaneous albinism IA; tumor rejection antigen AB
UniProt Protein Name
Tyrosinase
UniProt Gene Name
TYR
UniProt Entry Name
TYRO_HUMAN
Similar Products
Product Notes
The TYR tyr (Catalog #AAA77051) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Tyrosinase, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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