Bcl-2 Active Protein | BCL2 active protein

Bcl-2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA257842

• Gene Names: BCL2; Bcl-2; PPP1R50
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Bcl-2; N/A; Bcl-2 Protein, Human, Recombinant (His Tag); Human BCL2/Bcl-2 Protein (His Tag); Bcl-2 Protein, Human; PPP1R50 Protein, Human; B-cell CLL/lymphoma 2; BCL2 active protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 20% glycerol, 100mM Arg, pH8.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp211

• Species: Human
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human BCL2 isoform 1 (P10415-1)(Met 1-Asp 211) was expressed, with a polyhistide tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human BCL2-His at 10ug/ml (100uL/well) can bind biotinylated mouse BCL2L1-His. The EC50 of biotinylated mouse BCL2L1-His is 0.07-0.15ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized human BCL2-His at 10 ug/ml (100 ul/well) can bind biotinylated mouse BCL2L1-His, The EC50 of biotinylated mouse BCL2L1-His is 0.07-0.15 ug/ml.)

SDS-PAGE

Related Product Information for BCL2 active protein

Background: BCL2 (B-cell leukemia/lymphoma 2, N-Histidine-tagged), also known as Bcl-2, belongs to the Bcl-2 family. Bcl-2 family proteins regulate and contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four BH (bcl-2 homology) domains. Certain members such as Bcl-2, Bcl-xl and Mcl1 are anti-apoptotic, whilst others are pro-apoptotic. Most Bcl-2 family members contain a C-terminal transmembrane domain that functions to target these proteins to the outer mitochondrial and other intracellular membranes. It is expressed in a variety of tissues. BCL2 blocks the apoptotic death of some cells such as lymphocytes. It also regulates cell death by controlling the mitochondrial membrane permeability and inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

Product Categories/Family for BCL2 active protein

Cancer Drug Targets; Death Receptor Signaling

References

Tsujimoto Y, et al. (1984) Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18) chromosome translocation. Science. 226(4678):1097-99.
Cleary ML, et al. (1986) Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation. Cell. 47(1):19-28.
Otake Y, et al. (2007) Overexpression of nucleolin in chronic lymphocytic leukemia cells induces stabilization of Bcl-2/Bcl-2 mRNA. Blood. 109(7):3069-75.

NCBI and Uniprot Product Information

• NCBI GI #: 72198189
• NCBI GeneID: 596
• NCBI Accession #: NP_000624.2
• NCBI GenBank Nucleotide #: NM_000633.2
• UniProt Accession #: P10415; P10416; Q13842; Q16197
• Molecular Weight: 26,266 Da
• NCBI Official Full Name: apoptosis regulator Bcl-2 alpha isoform
• NCBI Official Synonym Full Names: B-cell CLL/lymphoma 2
• NCBI Official Symbol: BCL2
• NCBI Official Synonym Symbols: Bcl-2; PPP1R50
• NCBI Protein Information: apoptosis regulator Bcl-2; protein phosphatase 1, regulatory subunit 50
• UniProt Protein Name: Apoptosis regulator Bcl-2
• UniProt Gene Name: BCL2
• UniProt Entry Name: BCL2_HUMAN