Carbonic Anhydrase II Active Protein | CA-II active protein

Recombinant Human Carbonic Anhydrase II Protein

Cat. Num. AAA281421

• Gene Names: CA2; CAC; CAII; Car2; CA-II; HEL-76; HEL-S-282
• Purity: >90% by SDS-PAGE.
• Synonyms: Carbonic Anhydrase II; N/A; Recombinant Human Carbonic Anhydrase II Protein; CAC; CAII; Car2; HEL-76; HEL-S-282; CA-II active protein

• Host: E Coli
• Purity/Purification: >90% by SDS-PAGE.
• Form/Format: Lyophilized from a 0.22 um filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
• Sequence Length: 260

• Species: Human
• Endotoxin: < 0.1 EU/ug of the protein by LAL method.
• Biological Activity: Measured by its esterase activity. The specific activity is >840 pmoles/min/ug, as measured with 1 mM 4-Nitrophenyl acetate and 0.1 ug enzyme at 400 nm in 100 ?L of 12.5 mM Tris, 75 mM NaCl, pH 7.5
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
• Preparation and Storage: Store the lyophilized protein at -20°C to -80°C for long term.; After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. Avoid repeated freeze/thaw cycles.

SDS-PAGE

(Recombinant Human Carbonic anhydrase II Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 30 kDa.)

Related Product Information for CA-II active protein

Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification.

Product Categories/Family for CA-II active protein

Other Recombinant Protein

NCBI and Uniprot Product Information

• NCBI GI #: 115456
• NCBI GeneID: 760
• UniProt Accession #: P00918
• NCBI Official Full Name: Carbonic anhydrase 2
• NCBI Official Synonym Full Names: carbonic anhydrase 2
• NCBI Official Symbol: CA2
• NCBI Official Synonym Symbols: CAC; CAII; Car2; CA-II; HEL-76; HEL-S-282
• NCBI Protein Information: carbonic anhydrase 2
• UniProt Protein Name: Carbonic anhydrase 2
• UniProt Gene Name: CA2
• UniProt Synonym Gene Names: CAC; CA-II
• UniProt Entry Name: CAH2_HUMAN