WB (Western Blot)
(Anti-CA2 rabbit polyclonal antibody at 1:500 dilutionLane A: HEK293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:30 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human Carbonic anhydrase II Polyclonal Antibody | anti-CA2 antibody
Anti-Carbonic anhydrase II Antibody, Rabbit Polyclonal
Gene Names
CA2; CAC; CAII; Car2; CA-II; HEL-76
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Carbonic anhydrase II, Antibody; Anti-Carbonic anhydrase II Antibody, Rabbit Polyclonal; Carbonic Anhydrase II/CA2 Antibody, Rabbit PAb, Antigen Affinity Purified; carbonic anhydrase II; Anti-CA-II Antibody; Anti-CAC Antibody; Anti-CAII Antibody; Anti-Car2 Antibody; Anti-HEL-76 Antibody; Anti-HEL-S-282 Antibody; anti-CA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Carbonic Anhydrase II/CA2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CA2 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Human Carbonic Anhydrase II/CA2 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase II/CA2 (rh Carbonic Anhydrase II/CA2). Carbonic Anhydrase II/CA2 specific IgG was purified by Human Carbonic Anhydrase II/CA2 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-CA2 rabbit polyclonal antibody at 1:500 dilutionLane A: HEK293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:30 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-CA2 rabbit polyclonal antibody at 1:500 dilutionLane A: HEK293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:30 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(CA2 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg Caco-2 Whole Cell Lysate2 uL anti-CA2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CA2 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size: 29 kDa)
IP (Immunoprecipitation)
(CA2 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg Caco-2 Whole Cell Lysate2 uL anti-CA2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CA2 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size: 29 kDa)
Related Product Information for anti-CA2 antibody
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human alpha carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
References
Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20>Lilias A, et al. (1972) Crystal Structure of Human Carbonic Anhydrase C. Nature new biology. 235: 131-7.Li XJ, et al. (2002) Carbonic Anhydrase II Binds to and Enhances Activity of the Na+/H+ Exchanger. The Journal of Biological Chemistry. 277: 36085-91.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,246 Da
NCBI Official Full Name
carbonic anhydrase 2 isoform 1
NCBI Official Synonym Full Names
carbonic anhydrase II
NCBI Official Symbol
CA2
NCBI Official Synonym Symbols
CAC; CAII; Car2; CA-II; HEL-76
NCBI Protein Information
carbonic anhydrase 2; carbonate dehydratase II; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; epididymis luminal protein 76
UniProt Protein Name
Carbonic anhydrase 2
UniProt Gene Name
CA2
UniProt Synonym Gene Names
CAC; CA-II
UniProt Entry Name
CAH2_HUMAN
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Product Notes
The CA2 ca2 (Catalog #AAA254956) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Carbonic anhydrase II Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic anhydrase II can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CA2 ca2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic anhydrase II, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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