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Application Data
Interleukin 24 (IL24) Active Protein | IL24 active protein
Active Interleukin 24 (IL24)
Gene Names
IL24; C49A; FISP; MDA7; MOB5; ST16; IL10B
Reactivity
Homo sapiens (Human)
Applications
Invitro Assay, Activity Assay, Cell Culture
Purity
>92%
Synonyms
Interleukin 24 (IL24); N/A; Active Interleukin 24 (IL24); C49A; FISP; IL10B; MDA7; Mob-5; ST16; Mda-7; Melanoma Differentiation Association Protein 7; Suppression Of Tumorigenicity 16; IL24 active protein
Host
E. coli
Reactivity
Homo sapiens (Human)
Purity/Purification
>92%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% sarcosyl and 5% trehalose.
Applicable Applications for IL24 active protein
Invitro Assay, Activity Assay, Cell Culture
Source
Prokaryotic expression
Residues
Gly51~Leu206
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
8.6
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Application Data
Application Data
Application Data
Application Data
WB (Western Blot)
(Sample: Recombinant IL24, Human; Antibody: Rabbit Anti-Human IL24 Ab)
WB (Western Blot)
(Sample: Recombinant IL24, Human; Antibody: Rabbit Anti-Human IL24 Ab)
SDS-PAGE
(Sample: Active recombinant IL24, Human)
SDS-PAGE
(Sample: Active recombinant IL24, Human)
Activity
(IL24 (interleukin 24) is a cytokine that belongs to IL10 family. This protein can induce apoptosis selectively in various cancer cells, including ECV304. Thus, inhibition of cell proliferation assay of IL24 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL24. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Inhibition of ECV-304 cells proliferation after incubation with IIL24 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with various concentrations of IL24 for 48h. The mean OD value of ECV-304 assessed by CCK-8 was shown in Figure 2. It was obvious that IL24 significantly decreased cell viability of ECV-304 cells.)
Activity
(IL24 (interleukin 24) is a cytokine that belongs to IL10 family. This protein can induce apoptosis selectively in various cancer cells, including ECV304. Thus, inhibition of cell proliferation assay of IL24 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL24. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Inhibition of ECV-304 cells proliferation after incubation with IIL24 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with various concentrations of IL24 for 48h. The mean OD value of ECV-304 assessed by CCK-8 was shown in Figure 2. It was obvious that IL24 significantly decreased cell viability of ECV-304 cells.)
Product Categories/Family for IL24 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 19.5kDa
Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions
Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions
NCBI Official Full Name
interleukin-24 isoform 3
NCBI Official Synonym Full Names
interleukin 24
NCBI Official Symbol
IL24
NCBI Official Synonym Symbols
C49A; FISP; MDA7; MOB5; ST16; IL10B
NCBI Protein Information
interleukin-24
UniProt Protein Name
Interleukin-24
UniProt Gene Name
IL24
UniProt Synonym Gene Names
MDA7; ST16; IL-24; MDA-7
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Product Notes
The IL24 il24 (Catalog #AAA148127) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Interleukin 24 (IL24) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 24 (IL24) can be used in a range of immunoassay formats including, but not limited to, Invitro Assay, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the IL24 il24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 24 (IL24), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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