WB (Western Blot)
(Figure 4. Western BlotSample: Recombinant LMTK3, Human;Antibody: Rabbit Anti-Human LMTK3 Ab)
Lemur Tyrosine Kinase 3 (LMTK3) Active Protein | LMTK3 active protein
Active Lemur Tyrosine Kinase 3 (LMTK3)
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Figure 4. Western BlotSample: Recombinant LMTK3, Human;Antibody: Rabbit Anti-Human LMTK3 Ab)
WB (Western Blot)
(Figure 4. Western BlotSample: Recombinant LMTK3, Human;Antibody: Rabbit Anti-Human LMTK3 Ab)
SDS_PAGE
(Figure 3. SDS-PAGESample: Active recombinant LMTK3, Human)
SDS_PAGE
(Figure 3. SDS-PAGESample: Active recombinant LMTK3, Human)
Application Data
(Cell proliferation was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with various concentrations of LMTK3 for 72h. The dose-effect curve of LMTK3 was shown in Figure 2. It was obvious that LMTK3 significantly promoted cell proliferation of MCF-7 cells. The ED50 for this effect is approximately 6.14~50ng/mL.)
Application Data
(Cell proliferation was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with various concentrations of LMTK3 for 72h. The dose-effect curve of LMTK3 was shown in Figure 2. It was obvious that LMTK3 significantly promoted cell proliferation of MCF-7 cells. The ED50 for this effect is approximately 6.14~50ng/mL.)
Bioactivity
(Mechanism:Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance. It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line. To test the bioactivity of LMTK3, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of LMTK3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C.Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.)
Bioactivity
(Mechanism:Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance. It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line. To test the bioactivity of LMTK3, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of LMTK3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C.Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.)
Sequence
Sequence
NCBI and Uniprot Product Information
Accurate Molecular Mass: 46kDa as determined by SDS-PAGE reducing conditions.
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Product Notes
The LMTK3 lmtk3 (Catalog #AAA146624) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Lemur Tyrosine Kinase 3 (LMTK3) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Lemur Tyrosine Kinase 3 (LMTK3) can be used in a range of immunoassay formats including, but not limited to, Invitro Assay, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the LMTK3 lmtk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lemur Tyrosine Kinase 3 (LMTK3), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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