WB (Western Blot)
(Figure 1. Western blot analysis of MASP2 using anti-MASP2 antibody.)
Rabbit anti-Human MASP2 Polyclonal Antibody | anti-MASP2 antibody
Anti-MASP2 Antibody
Gene Names
MASP2; sMAP; MAP19; MASP-2; MASP1P1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
MASP2, Antibody; Anti-MASP2 Antibody; Mannan-binding lectin serine protease 2; MBL-associated serine protease 2; Mannose-binding protein-associated serine protease 2; MASP-2; MASP2; anti-MASP2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
686
Applicable Applications for anti-MASP2 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence of human MASP2 (EAGQYGVYTKVINYIPWIENIISDF).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Figure 1. Western blot analysis of MASP2 using anti-MASP2 antibody.)
WB (Western Blot)
(Figure 1. Western blot analysis of MASP2 using anti-MASP2 antibody.)
FCM/FACS (Flow Cytometry)
FCM/FACS (Flow Cytometry)
FCM/FACS (Flow Cytometry)
FCM/FACS (Flow Cytometry)
Related Product Information for anti-MASP2 antibody
Description: Rabbit IgG polyclonal antibody for MASP2 detection. Tested with WB, FCM in Human.
Background: Mannan-binding lectin serine protease 2 is a protein that in humans is encoded by the MASP2 gene. This gene is mapped to 1p36.22. This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease. This protease cleaves complement components C2 and C4 in order to generate C3 convertase in the lectin pathway of the complement system. The encoded protease also plays a role in the coagulation cascade through cleavage of prothrombin to form thrombin. Myocardial infarction and acute stroke patients exhibit reduced serum concentrations of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Background: Mannan-binding lectin serine protease 2 is a protein that in humans is encoded by the MASP2 gene. This gene is mapped to 1p36.22. This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease. This protease cleaves complement components C2 and C4 in order to generate C3 convertase in the lectin pathway of the complement system. The encoded protease also plays a role in the coagulation cascade through cleavage of prothrombin to form thrombin. Myocardial infarction and acute stroke patients exhibit reduced serum concentrations of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
References
1. Stover, C. M., Schwaeble, W. J., Lynch, N. J., Thiel, S., Speicher, M. R. Assignment of the gene encoding mannan-binding lectin-associated serine protease 2 (MASP2) to human chromosome 1p36.3-p36.2 by in situ hybridization and somatic cell hybrid analysis. Cytogenet. Cell Genet. 84: 148-149, 1999.
2. Stover, C. M., Thiel, S., Thelen, M., Lynch, N. J., Vorup-Jensen, T., Jensenius, J. C., Schwaeble, W. J. Two constituents of the initiation complex of the mannan-binding lectin activation pathway of complement are encoded by a single structural gene. J. Immun. 162: 3481-3490, 1999.
3. Takahashi, M., Endo, Y., Fujita, T., Matsushita, M. A truncated form of mannose-binding lectin-associated serine protease (MASP)-2 expressed by alternative polyadenylation is a component of the lectin complement pathway. Int. Immun. 11: 859-863, 1999.
2. Stover, C. M., Thiel, S., Thelen, M., Lynch, N. J., Vorup-Jensen, T., Jensenius, J. C., Schwaeble, W. J. Two constituents of the initiation complex of the mannan-binding lectin activation pathway of complement are encoded by a single structural gene. J. Immun. 162: 3481-3490, 1999.
3. Takahashi, M., Endo, Y., Fujita, T., Matsushita, M. A truncated form of mannose-binding lectin-associated serine protease (MASP)-2 expressed by alternative polyadenylation is a component of the lectin complement pathway. Int. Immun. 11: 859-863, 1999.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mannan-binding lectin serine protease 2 isoform 1 preproprotein
NCBI Official Synonym Full Names
mannan binding lectin serine peptidase 2
NCBI Official Symbol
MASP2
NCBI Official Synonym Symbols
sMAP; MAP19; MASP-2; MASP1P1
NCBI Protein Information
mannan-binding lectin serine protease 2
UniProt Protein Name
Mannan-binding lectin serine protease 2
UniProt Gene Name
MASP2
UniProt Synonym Gene Names
MASP-2
UniProt Entry Name
MASP2_HUMAN
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Product Notes
The MASP2 masp2 (Catalog #AAA125449) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MASP2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MASP2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the MASP2 masp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MASP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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