Perforin 1 (PRF1) Active Protein | PRF1 active protein

Active Perforin 1 (PRF1)

Cat. Num. AAA152021

• Gene Names: PRF1; P1; PFP; FLH2; PFN1; HPLH2
• Reactivity: Homo sapiens (Human)
• Applications: Activity Assay, Cell Culture
• Purity: >80%
• Synonyms: Perforin 1 (PRF1); N/A; Active Perforin 1 (PRF1); PRF1/Perforin 1; FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein; PRF1 active protein

• Host: E. coli
• Reactivity: Homo sapiens (Human)
• Purity/Purification: >80%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl, 5% Trehalose.
• Concentration: Original Concentration: 350ug/mL (varies by lot)

• Applicable Applications for PRF1 active protein: Activity Assay, Cell Culture
• Source: Prokaryotic expression.
• Residues: Pro22~Trp555
• Tags: N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method).
• Predicted isoelectric point: 7.8
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS_PAGE

Gene Sequencing

(Gene Sequencing (extract))

Bioactivity

(Perforin 1 (PRF1) is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin binds to the target cell's plasma membrane, and oligomerises in a Ca2+ dependent manner to form pores on the target cell. The pore formed allows for the passive diffusion of a family of pro-apoptotic proteases, known as the granzymes, into the target cell. The activity of recombinant PRF1 was measured by lysis of erythrocytes using a hemolysis assay. A general procedure is as fllows: two-fold dilute the recombinant human PRF1 with 0.9% NaCl, add 50ul a serial dilution of PRF1, 10ul 0.1M CaCl2 to each well, then add 50ul 0.25% rabbit erythrocyte (RaE) to each well and mixed gently. Add 50ul 0.9% NaCl to reaplace PRF1 in control wells. The plate is incubated for 20 hours at 37 degree C, 5% CO2. The results are shown in Figure 2. It was obvious that the minimal effective concentration of PRF1 is 2.5ug/ml.Figure 1. Hemolysis activity of recombinant human PRF1(A) 0.25% RaE treated with 2.5ug/ml PRF1 for 20h;(B) 0.25% RaE treated with without PRF1.)

Sequence

Product Categories/Family for PRF1 active protein

Immune molecule; Active proteins/AP

NCBI and Uniprot Product Information

• NCBI GI #: 133908621
• NCBI GeneID: 5551
• NCBI Accession #: NP_001076585.1
• NCBI GenBank Nucleotide #: NM_001083116.1
• UniProt Accession #: P14222; Q59F57; Q86WX7; B2R6X4
• Molecular Weight: Predicted Molecular Mass: 62.9kDa; Accurate Molecular Mass: 63kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: perforin-1
• NCBI Official Synonym Full Names: perforin 1 (pore forming protein)
• NCBI Official Symbol: PRF1
• NCBI Official Synonym Symbols: P1; PFP; FLH2; PFN1; HPLH2
• NCBI Protein Information: perforin-1; cytolysin; lymphocyte pore forming protein; lymphocyte pore-forming protein
• UniProt Protein Name: Perforin-1
• UniProt Gene Name: PRF1
• UniProt Synonym Gene Names: PFP; P1; PFP
• UniProt Entry Name: PERF_HUMAN