FCM/FACS (Flow Cytometry)
(Separation of human perforin positive CD56 positive NK cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-Perforin (d9G) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
anti-Human, Cow Perforin Antibody | anti-PRF1 antibody
Anti-Perforin PE-Cy7
Gene Names
PRF1; P1; PFP; FLH2; PFN1; HPLH2
Reactivity
Human, Cow
Applications
Flow Cytometry, Functional Assay
Synonyms
Perforin; N/A; Anti-Perforin PE-Cy7; perforin 1; PRF1; P1; PFP; HPLH2; anti-PRF1 antibody
Reactivity
Human, Cow
Isotype
Mouse IgG2b kappa
Clone Number
dG9
Specificity
The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
Form/Format
PE-Cy7
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffe
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffe
Applicable Applications for anti-PRF1 antibody
FCM/FACS (Flow Cytometry)
Immunogen
purified granules from human YT lymphoma cell line
Negative species
Mouse
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human perforin positive CD56 positive NK cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-Perforin (d9G) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Separation of human perforin positive CD56 positive NK cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-Perforin (d9G) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD56 (LT56) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood) and intracellular staining of human lymphocytes using anti-Perforin (d9G) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD56 (LT56) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood) and intracellular staining of human lymphocytes using anti-Perforin (d9G) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Perforin (d9G) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Perforin (d9G) PE-Cy™7 antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)
Related Product Information for anti-PRF1 antibody
Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.
References
*Vargas-Inchaustegui DA, Demberg T, Robert-Guroff M: A CD8α(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities. Immunology. 2011 Nov;134(3):326-40., URL: http://www.ncbi.nlm.nih.gov/pubmed/21978002,*Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. J Mol Cell Biol. 2010 Jun;2(3):139-51., URL: http://www.ncbi.nlm.nih.gov/pubmed/20348186,*Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW: Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions. PLoS Pathog. 2012;8(11):e1002984., URL: http://www.ncbi.nlm.nih.gov/pubmed/23144609,*Hersperger AR, Makedonas G, Betts MR: Flow cytometric detection of perforin upregulation in human CD8 T cells. Cytometry A. 2008 Nov;73(11):1050-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/18615597,*Hameed A, Olsen KJ, Cheng L, Fox WM, Hruban RH, Podack ER: Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am J Pathol. 1992 May;140(5):1025-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/1374586,
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,377 Da
NCBI Official Full Name
perforin-1
NCBI Official Synonym Full Names
perforin 1 (pore forming protein)
NCBI Official Symbol
PRF1
NCBI Official Synonym Symbols
P1; PFP; FLH2; PFN1; HPLH2
NCBI Protein Information
perforin-1; cytolysin; lymphocyte pore forming protein; lymphocyte pore-forming protein
UniProt Protein Name
Perforin-1
UniProt Gene Name
PRF1
UniProt Synonym Gene Names
PFP; P1; PFP
UniProt Entry Name
PERF_HUMAN
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Product Notes
The PRF1 prf1 (Catalog #AAA129204) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Perforin PE-Cy7 reacts with Human, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Perforin can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the PRF1 prf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Perforin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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