Interleukin-18 Active Protein | IL-18 active protein
Human Interleukin-18 Recombinant
Purity
>92%, as determined by SDS-PAGE and HPLC
Synonyms
Interleukin-18; N/A; Human Interleukin-18 Recombinant; IGIF; IL1F4; IL18; il-18; IL-18 active protein
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Purity/Purification
>92%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Interleukin-18 was lyophilized from 0.2 um filtered PBS solution pH 7.4.
Sequence Length
189
Source Note
Optimized DNA sequence encoding Human Interleukin-18 mature chain was expressed in Escherichia Coli
Presentation
Recombinant Interleukin-18 was lyophilized from 0.2 um filtered PBS solution pH 7.4.
Biological Activity
The ED(50) was determined by the dose-dependent proliferation ofhuman PBMC co-stimulated with IL-12 cells was found to be in the range of 5ng/ml.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Molecular Weight Note
Native human Interleukin-18, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 18 kDa. Recombinant Interleukin-18 is a disulfide-linked monomer protein consisting of 158 amino acid residue subunits, migrates as an approximately 18 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 1 year from date of receipt at -20°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2°-8°C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Product Categories/Family for IL-18 active protein
References
• IL-18 binding protein-expressing mesenchymal stem cells improve myocardial protection after ischemia or infarction .PNAS, Sep 2009; 10.1073/pnas.0908924106.
• IL-18 and urinary ngal predict dialysis and graft recovery after kidney transplantation .J. Am. Soc. Nephrol., Sep 2009; 10.1681/ASN.2009030264.
• a functional polymorphism in IL-18 is associated with severity of bronchial asthma .Am. J. Respir. Crit. Care Med., Sep 2009; 10.1164/rccm.200905-0652 degree C.
• il-12p40 and IL-18 play pivotal roles in orchestrating the cell-mediated immune response to a poxvirus infection .J. Immunol., Sep 2009; 183: 3324 - 3331.
• neutrophil chemokines and their role in IL-18-mediated increase in neutrophil o2– production and intestinal edema following alcohol intoxication and burn injury .Am J Physiol Gastrointest Liver Physiol, Aug 2009; 297: G340 - G347.
• IL-18 and urinary ngal predict dialysis and graft recovery after kidney transplantation .J. Am. Soc. Nephrol., Sep 2009; 10.1681/ASN.2009030264.
• a functional polymorphism in IL-18 is associated with severity of bronchial asthma .Am. J. Respir. Crit. Care Med., Sep 2009; 10.1164/rccm.200905-0652 degree C.
• il-12p40 and IL-18 play pivotal roles in orchestrating the cell-mediated immune response to a poxvirus infection .J. Immunol., Sep 2009; 183: 3324 - 3331.
• neutrophil chemokines and their role in IL-18-mediated increase in neutrophil o2– production and intestinal edema following alcohol intoxication and burn injury .Am J Physiol Gastrointest Liver Physiol, Aug 2009; 297: G340 - G347.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
interleukin-18 isoform 2 proprotein
UniProt Protein Name
Interleukin-18
UniProt Gene Name
IL18
UniProt Synonym Gene Names
IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma
UniProt Entry Name
IL18_HUMAN
Similar Products
Product Notes
The IL-18 il18 (Catalog #AAA14421) is an Active Protein produced from E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Interleukin-18, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.