PAI-1 active protein

Human PAI-1

Cat. Num. AAA79255

• Gene Names: SERPINE1; PAI; PAI1; PAI-1; PLANH1
• Reactivity: Human
• Purity: > 95% by SDS-PAGE & HPLC analyses
• Synonyms: PAI-1; N/A; Human PAI-1; Plasminogen Activator Inhibitor-1; Serpin E1; PAI-1 active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 95% by SDS-PAGE & HPLC analyses
• Form/Format: Lyophilized (50mM Sodium Acetate, pH 5.5 + 100mM NaCI)
• Sequence: VHHPPSYVAH LASDFGVRVF QQVAQASKDR NVVFSPYGVA SVLAML QLTT GGETQQQIQA AMGFKIDDKG MAPALRHLYK ELMGPWNKDE I STTDAIFVQ RDLKLVQGFM PHFFRLFRST VKQVDFSEVE RARFIIN DWV KTHTKGMISN LLGKGAVDQL TRLVLVNALY FNGQWKTPFP DS STHRRLFH KSDGSTVSVP MMAQTNKFNY TEFTTPDGHY YDILELPY HG DTLSMFIAAP YEKEVPLSAL TNILSAQLIS HWKGNMTRLP RLL VLPKFSL ETEVDLRKPL ENLGMTDMFR QFQADFTSLS DQEPLHVAQ A LQKVKIEVNE SGTVASSSTA VIVSARMAPE EIIMDRPFLF VVRH NPTGTV LFMGQVMEP
• Sequence Length: 402

• Endotoxin Level: < 0.1 ng/ug of protein (<1EU/ug).
• Biological Activity: Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37°C. Half maximal inhibition against 1.0 ug/ml of single chain tPA was obtained at a concentration of 2.0 ug/ml.
• Length (aa): 379
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/m. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Preparation and Storage: The lyophilized protein is stable at room temperature for 1 month and at 4°C for 3 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

Related Product Information for PAI-1 active protein

Plasminogen Activator Inhibitor-1 (PAI-1, Serpin E1) is a member of the serpin family of serine protease inhibitors, and is the primary inhibitor of urokinase and tissue plasminogen activator (tPA). PAI-1 is expressed predominantly in adipose, liver and vascular tissues, and is also produced by certain tumor cells. Elevated levels of PAI-1 are associated with obesity, diabetes and cardiovascular disease, and increased production of PAI-1 is induced by various obesity related factors such as TNFalpha, glucose, insulin, and very-low-density lipoprotein. The obesity related elevation of PAI-1 levels along with the consequential deficiency in plasminogen activators can lead directly to increased risk of thrombosis and other coronary diseases. Accordingly, PAI-1 has been implicated as an important molecular link between obesity and coronary disease. PAI-1 can also specifically bind vitronectin (VTN) to form a stable active complex with an increased circulatory half life relative to free PAI-1. Recombinant human PAI-1 is a 42.7 kDa protein containing 379 amino acid residues.

Product Categories/Family for PAI-1 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 10835159
• NCBI GeneID: 5054
• NCBI Accession #: NP_000593.1
• NCBI GenBank Nucleotide #: NM_000602.4
• UniProt Accession #: P05121; B7Z4S0; F8WD53
• Molecular Weight: 42.7 kDa
• NCBI Official Full Name: plasminogen activator inhibitor 1
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• NCBI Official Symbol: SERPINE1
• NCBI Official Synonym Symbols: PAI; PAI1; PAI-1; PLANH1
• NCBI Protein Information: plasminogen activator inhibitor 1; serpin E1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• UniProt Protein Name: Plasminogen activator inhibitor 1
• UniProt Gene Name: SERPINE1
• UniProt Synonym Gene Names: PAI1; PLANH1; PAI; PAI-1
• UniProt Entry Name: PAI1_HUMAN