Application Data
(Fig. 3: Confluent PAE/KDR cells were stimulated with 50ng/ml human VEGF165 for 10 min at 37°C. Cells were lysed and an IP was performed using a mouse anti-human KDR antibody (Cat# WB was performed with a mouse anti-human KDR antibody (Cat# and an anti- Phosphotyrosine antibody.)
VEGF165 active protein
Human VEGF165
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human
Purity
> 95% by SDS-PAGE & silver stain
Synonyms
VEGF165; N/A; Human VEGF165; VEGF-A; VPF; VEGF165 active protein
Host
E Coli
Reactivity
Human
Purity/Purification
> 95% by SDS-PAGE & silver stain
Form/Format
Sterile filtered protein solution was lyophilized from 50 mM acetic acid, free from stabilizers and preservatives.
Sequence Positions
APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
Sequence
N-Terminal Sequence: APMAEGG
Sequence Length
165
Amino Acids
165 aa
Reconstitution
The lyophilized VEGF165 should be reconstituted in 50 mM acetic acid to a concentration not lower than 50 ug/ml. Store at 2°C to 8°C for up to 1 week or prepare for extended storage.
Buffer
50 mM acetic acid
Preparation and Storage
After initial reconstitution, the addition of at least 0.1% human or bovine serum albumin is recommended for frozen aliquots. Prepare and store working aliquots at -20°C to -80°C.
| Product Form | Temperature | Storage Time |
|---|---|---|
| Lyophilized | -20°C to -80°C | 1 year |
| Lyophilized | 2°C to 8°C | 6 months |
| Lyophilized | Room Temperature | 1 month |
| Extended Storage | -20°C to -80°C | 3 months |
