Human VEGF165 ELISA Kit | VEGF165 elisa kit

Human VEGF165 ELISA Kit

Cat. Num. AAA258418

• Reactivity: Human
• Synonyms: VEGF165; N/A; Human VEGF165 ELISA Kit; VEGF165 ELISA Kit, Human; vascular endothelial growth factor A; MVCD1 ELISA Kit, Human; VEGF ELISA Kit, Human; VPF ELISA Kit, Human; VEGF165 elisa kit

• Reactivity: Human
• Specificity: For the quantitative determination of Human VEGF165 concentration in serum, cell culture supernates and plasma.
• Assay Type: Solid Phase Sandwich ELISA (quantitative)
• Sample Types: Serum, Cell supernatant, Plasma
• Detection Range: 78.13-5000pg/mL
• Sensitivity: 41.06pg/mL
• Conjugation: HRP
• Interference: Preparations of the factors listed below at 50ng/mL in a mid-range recombinant human VEGF165 control were assayed for interference. No significant interference was observed.Interference was observed with recombinant human VEGFR1 and Mouse VEGFR1 at concentrations >= 0.5ng/mL, and with recombinant Mouse VEGF164 at concentrations >= 5.0ng/mL.
• Materials Provided: 1. 96 well microplate coated with Capture Antibody; 2. Detection Antibody conjugated to HRP; 3. Standards; 4. Wash Buffer Concentrate; 5. Dilution Buffer Concentrate; 6. Color Reagent A; 7. Color Reagent B; 8. Stop Solution

Application Data

(This assay recognizes both recombinant and natural Human VEGF165. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)

Standard Curve (Sample)

(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)

Related Product Information for VEGF165 elisa kit

This VEGF165 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGFA protein in Serum, Cell supernatant, Plasma. It contains recombinant Human VEGFA, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.

Background: Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.

References

Woolard J. et al. (2004) VEGF165b, an inhibitory vascular endothelial growth factor splice variant: mechanism of action, in vivo effect on angiogenesis and endogenous protein expression. Cancer Res. 64(21): 7822-7835.
Jia SF, et al. (2008) VEGF165 is necessary to the metastatic potential of Fas(-) osteosarcoma cells but will not rescue the Fas(+) cells. J Exp Ther Oncol. 7(2): 89-97.
Cimpean AM, et al. (2008) Vascular endothelial growth factor A (VEGF A) as individual prognostic factor in invasive breast carcinoma. Rom J Morphol Embryol. 49(3): 303-8.
Hamdollah Zadeh MA, et al. (2008) VEGF-mediated elevated intracellular calcium and angiogenesis in human microvascular endothelial cells in vitro are inhibited by dominant negative TRPC6.
Microcirculation. 15(7): 605-14.
Eisenach PA, et al. (2010) MT1-MMP regulates VEGF-A expression through a complex with VEGFR-2 and Src. J Cell Sci. 123(Pt 23):4182-4193.
Claesson-Welsh L (2010) Gremlin: vexing VEGF receptor agonist. Blood. 116(18):3386-7.