Interleukin-36 alpha/IL-36 alpha Active Protein | IL-36alpha active protein

Recombinant Human Interleukin-36 alpha/IL-36 alpha

Cat. Num. AAA177939

• Gene Names: IL36A; FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: Interleukin-36 alpha/IL-36 alpha; N/A; Recombinant Human Interleukin-36 alpha/IL-36 alpha; Interleukin-36 Alpha; FIL1 Epsilon; Interleukin-1 Epsilon; IL-1 Epsilon; Interleukin-1 Family Member 6; IL-1F6; IL36A; FIL1E; IL1E; IL1F6; IL-36alpha active protein

• Host: E Coli
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of 50mM Tris-HCl, 150mM NaCl, 1 mM EDTA, 0.02% Tween 20, pH8.0.
• Sequence: Lys6-Phe158

• Species: Human
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Human Interleukin-36 Alpha is produced by our E Coli expression system and the target gene encoding Lys6-Phe158 is expressed.
• Activity: Measured in a cell proliferation assay using A-431 Human epithelial carcinoma cells. The ED50 for this effect is 12.0ng/ml.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

Bioactivity

SDS-PAGE

Related Product Information for IL-36alpha active protein

Human Interleukin-36alpha (IL-36alpha) is a secreted cytokine that belongs to the Interleukin 1 cytokine family. IL-36alpha is expressed in the immune system and the fetal brain, but not in other tissues or multiple hematopoietic cell lines. IL-36alpha is the only IL-1 family member found to be expressed on T-cells. IL-36alpha and IL-1F8 are involved in the regulation of adipose tissue gene expression. Importantly, IL-36alpha inhibits PPARgamma expression, which may lead to a reduction in adipocyte differentiation suggesting metabolic effects of this cytokine. IL-36alpha, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NFkappaB and MAPK in an IL-1Rrp2 dependent manner. This suggest that IL-36alpha may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

Product Categories/Family for IL-36alpha active protein

immunology

NCBI and Uniprot Product Information

• NCBI GI #: 7657092
• NCBI GeneID: 27179
• NCBI Accession #: NP_055255.1
• NCBI GenBank Nucleotide #: NM_014440.1
• UniProt Accession #: Q9UHA7; Q53SR7; Q5BLR4; Q7RTZ8; B2RAD9
• Molecular Weight: 17,684 Da
• NCBI Official Full Name: interleukin-36 alpha
• NCBI Official Synonym Full Names: interleukin 36, alpha
• NCBI Official Symbol: IL36A
• NCBI Official Synonym Symbols: FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
• NCBI Protein Information: interleukin-36 alpha; FIL1 epsilon; IL-1 epsilon; IL-1F6 (FIL-1-epsilon); interleukin 1 family, member 6 (epsilon); interleukin 1, epsilon; interleukin-1 epsilon; interleukin-1 family member 6
• UniProt Protein Name: Interleukin-36 alpha
• UniProt Gene Name: IL36A
• UniProt Synonym Gene Names: FIL1E; IL1E; IL1F6; IL-1 epsilon; IL-1F6
• UniProt Entry Name: IL36A_HUMAN