Leptin Active Protein | LEP active protein

Leptin Protein, Human, Recombinant

Cat. Num. AAA257847

• Gene Names: LEP; OB; OBS; LEPD
• Purity: >98% as determined by SDS-PAGE
• Synonyms: Leptin; N/A; Leptin Protein, Human, Recombinant; Human Leptin Protein; LEPD Protein, Human; OB Protein, Human; OBS Protein, Human; leptin; LEP active protein

• Host: E Coli
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Val22-Cys167

• Species: Human
• Activity: Measured by its binding ability in a functional ELISA.; 1. Immobilized human Leptin at 1.25ug/ml (100uL/well) can bind human Leptin receptor Fc chimera with a linear range of 0.032-4.0ug/ml.; 2. Immobilized human Leptin at 5ug/ml (100uL/well) can bind human Leptin receptor his with a linear range of 0.032-4.0ug/ml.
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the mature form of human Leptin (NP_000221.1)(Val 22-Cys 167) was expressed, with an additional Met.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Immobilized Human Leptin at 2 ug/mL (100 uL/well) can bind Human Leptin Receptor (His & hFc Tag), the EC50 of Human Leptin Receptor (His & hFc Tag) is 70-420 ng/mL.)

SDS-PAGE

Related Product Information for LEP active protein

Background: Leptin is one of the most important hormones secreted by adipocytes, as an adipokine that modulates multiple functions including energy homeostasis, thermoregulation, bone metabolism, endocrine, and pro-inflammatory immune responses. The circulating leptin levels serve as a gauge of energy stores, thereby directing the regulation of energy homeostasis, neuroendocrine function, and metabolism. Recent studies suggest that leptin is physiologically more important as an indicator of energy deficiency, rather than energy excess, and may mediate adaptation by driving increased food intake and directing neuroendocrine function to converse energy, such as inducing hypothalamic hypogonadism to prevent fertilization. One of these functions is the connection between nutritional status and immune competence. The adipocyte-derived hormone Leptin has been shown to regulate the immune response, innate, and adaptive response, both in normal and pathological conditions. Thus, Leptin is a mediator of the inflammatory response. Leptin has a dual effect on bone, acting by two independent mechanisms. As a signal molecule with growth factor characteristics, leptin can stimulate osteoblastic cells and inhibit osteoclast formation and activity, thus promoting osteogenesis. However, as a molecule that stimulates sympathetic neurons in the hypothalamus, leptin indirectly inhibits bone formation. This inhibitory effect of leptin mediated by activation of the sympathetic nervous system can be abrogated by the application of blood pressure-reducing beta-blockers, which also inhibit receptors of hypothalamic adrenergic neurons. Leptin appears to regulate some features defining Alzheimer's disease (AD) at the molecular and physiological level. Leptin can stimulate mitogenic and angiogenic processes in peripheral organs. Because leptin levels are elevated in obese individuals and excess body weight has been shown to increase breast cancer risk in postmenopausal women. Furthermore, a recent report clearly shows that targeting leptin signaling may reduce mammary carcinogenesis.

Product Categories/Family for LEP active protein

Neurotransmitter Associated Enzymes; AMPK Signaling Pathway

References

Surmacz E. (2007) Obesity hormone leptin: a new target in breast cancer Breast Cancer Res. 9(1): 301.Wodarski K, et al. (2009) Leptin as a modulator of osteogenesis. Ortop Traumatol Rehabil. 11(1): 1-6.Tezapsidis N, et al. (2009) Leptin: a novel therapeutic strategy for Alzheimer's disease. J Alzheimers Dis. 16(4): 731-40.Cai C, et al. (2009) Leptin in non-autoimmune inflammation. Inflamm Allergy Drug Targets. 8(4): 285-91.Fernndez-Riejos P, et al. (2010) Role of leptin in the activation of immune cells. Mediators Inflamm. 2010: 568343.Kelesidis T, et al. (2010) Narrative review: the role of leptin in human physiology: emerging clinical applications. Ann Intern Med. 152(2): 93-100.

NCBI and Uniprot Product Information

• NCBI GI #: 4557715
• NCBI GeneID: 3952
• NCBI Accession #: NP_000221.1
• NCBI GenBank Nucleotide #: NM_000230.2
• UniProt Accession #: P41159; O15158; Q56A88
• Molecular Weight: 18,641 Da
• NCBI Official Full Name: leptin
• NCBI Official Synonym Full Names: leptin
• NCBI Official Symbol: LEP
• NCBI Official Synonym Symbols: OB; OBS; LEPD
• NCBI Protein Information: leptin; obese protein; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse)
• UniProt Protein Name: Leptin
• UniProt Gene Name: LEP
• UniProt Synonym Gene Names: OB; OBS
• UniProt Entry Name: LEP_HUMAN